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Nitrogen Acquisition and Transport in the Ectomycorrhizal Symbiosis—Insights from the Interaction between an Oak Tree and Pisolithus tinctorius

2023-01Journal article Open access
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dc.contributor.authorSebastiana, Mónica
dc.contributor.authorSerrazina, Susana
dc.contributor.authorMonteiro, Filipa
dc.contributor.authorWipf, Daniel
dc.contributor.authorFromentin, Jérome
dc.contributor.authorTeixeira, Rita
dc.contributor.authorMalhó, Rui
dc.contributor.authorCourty, Pierre-Emmanuel
dc.date.accessioned2023-10-25T09:45:20Z
dc.date.available2023-10-25T09:45:20Z
dc.date.issued2023-01
dc.description.abstractIn temperate forests, the roots of various tree species are colonized by ectomycorrhizal fungi, which have a key role in the nitrogen nutrition of their hosts. However, not much is known about the molecular mechanisms related to nitrogen metabolism in ectomycorrhizal plants. This study aimed to evaluate the nitrogen metabolic response of oak plants when inoculated with the ectomycorrhizal fungus Pisolithus tinctorius. The expression of candidate genes encoding proteins involved in nitrogen uptake and assimilation was investigated in ectomycorrhizal roots. We found that three oak ammonium transporters were over-expressed in root tissues after inoculation, while the expression of amino acid transporters was not modified, suggesting that inorganic nitrogen is the main form of nitrogen transferred by the symbiotic fungus into the roots of the host plant. Analysis by heterologous complementation of a yeast mutant defective in ammonium uptake and GFP subcellular protein localization clearly confirmed that two of these genes encode functional am- monium transporters. Structural similarities between the proteins encoded by these ectomycorrhizal upregulated ammonium transporters, and a well-characterized ammonium transporter from E. coli, suggest a similar transport mechanism, involving deprotonation of NH4+, followed by diffusion of uncharged NH3 into the cytosol. This view is supported by the lack of induction of NH4+ detoxifying mechanisms, such as the GS/GOGAT pathway, in the oak mycorrhizal rootspt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSebastiana, M.; Serrazina, S.; Monteiro, F.; Wipf, D.; Fromentin, J.; Teixeira, R.; Malhó, R.; Courty, P.-E. Nitrogen Acquisition and Transport in the Ectomycorrhizal Symbiosis—Insights from the Interaction between an Oak Tree and Pisolithus tinctorius. Plants 2023, 12, 10pt_PT
dc.identifier.doi10.3390/plants12010010pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/29120
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationDL57/2016/CP[12345/2018]/CT[2475]pt_PT
dc.relationDL57/2016/CP[12345/2018]/CT[2477]pt_PT
dc.relationBiosystems and Integrative Sciences Institute
dc.relationBiosystems and Integrative Sciences Institute
dc.relationLinking Landscape, Environment, Agriculture and Food
dc.relationCentre for Ecology, Evolution and Environmental Changes
dc.relationPPBI-POCI-01-0145-FEDER-022122pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/journal/plantspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectmycorrhizapt_PT
dc.subjectnitrogenpt_PT
dc.subjectammonium transporterpt_PT
dc.subjectectomycorrhizal fungipt_PT
dc.subjectoak (cork oak)pt_PT
dc.titleNitrogen Acquisition and Transport in the Ectomycorrhizal Symbiosis—Insights from the Interaction between an Oak Tree and Pisolithus tinctoriuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardTitleLinking Landscape, Environment, Agriculture and Food
oaire.awardTitleCentre for Ecology, Evolution and Environmental Changes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04046%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04046%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04129%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00329%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FASP-AGR%2F0760%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.titlePlantspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationcd397b4c-17e6-40cc-8cdd-1dd1c0da5113
relation.isProjectOfPublication380fa69a-5155-4088-b81a-18976876e526
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relation.isProjectOfPublication.latestForDiscovery380fa69a-5155-4088-b81a-18976876e526

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