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Notch signaling in mouse blastocyst development and hatching

2020-06-02Journal article Open access
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dc.contributor.authorBatista, Mariana R.
dc.contributor.authorDiniz, Patrícia
dc.contributor.authorTorres, Ana
dc.contributor.authorde Moura Murta, Daniel
dc.contributor.authorLopes-da-Costa, Luís
dc.contributor.authorSilva, Elisabete
dc.date.accessioned2022-04-18T16:51:54Z
dc.date.available2022-04-18T16:51:54Z
dc.date.issued2020-06-02
dc.descriptionResearch Areas: Developmental Biologypt_PT
dc.description.abstractBackground: Mammalian early embryo development requires a well-orchestrated interplay of cell signaling pathways. Notch is a major regulatory pathway involved in cell-fate determination in embryonic and adult scenarios. However, the role of Notch in embryonic pre-implantation development is controversial. In particular, Notch role on blastocyst development and hatching remains elusive, and a complete picture of the transcription and expression patterns of Notch components during this time-period is not available. Results: This study provided a comprehensive view on the dynamics of individual embryo gene transcription and protein expression patterns of Notch components (receptors Notch1–4; ligands Dll1 and Dll4, Jagged1–2; and effectors Hes1–2), and their relationship with transcription of gene markers of pluripotency and differentiation (Sox2, Oct4, Klf4, Cdx2) during mouse blastocyst development and hatching. Transcription of Notch1–2, Jagged1–2 and Hes1 was highly prevalent and dynamic along stages of development, whereas transcription of Notch3–4, Dll4 and Hes2 had a low prevalence among embryos. Transcription levels of Notch1, Notch2, Jagged2 and Hes1 correlated with each other and with those of pluripotency and differentiation genes. Gene transcription was associated to protein expression, except for Jagged2, where high transcription levels in all embryos were not translated into protein. Presence of Notch signaling activity was confirmed through nuclear NICD and Hes1 detection, and downregulation of Hes1 transcription following canonical signaling blockade with DAPT. In vitro embryo culture supplementation with Jagged1 had no effect on embryo developmental kinetics. In contrast, supplementation with Jagged2 abolished Jagged1 transcription, downregulated Cdx2 transcription and inhibited blastocyst hatching. Notch signaling blockade by DAPT downregulated transcription of Sox2, and retarded embryo hatching. Conclusion: Transcription of Notch genes showed a dynamic pattern along blastocyst development and hatching. Data confirmed Notch signaling activity, and lead to the suggestion that Notch canonical signaling may be operating through Notch1, Notch3, Jagged1 and Hes1. Embryo culture supplementation with Jagged1 and Jagged2 unveiled a possible regulatory effect between Jagged1, Cdx2 and blastocyst hatching. Overall, results indicate that a deregulation in Notch signaling, either by its over or under-activation, affects blastocyst development and hatching.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBatista MR, Diniz P, Torres A, Murta D, Lopes-da-Costa L, Silva E. 2020. Notch signaling in mouse blastocyst development and hatching, BMC Developmental Biology, 20(1):9. Doi 10.1186/s12861-020-00216-2pt_PT
dc.identifier.doi10.1186/s12861-020-00216-2pt_PT
dc.identifier.issn1471-213X
dc.identifier.urihttp://hdl.handle.net/10400.5/24102
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBMCpt_PT
dc.relationUID/CVT/276/2013pt_PT
dc.relationNotch-Wnt signaling cross-talk in the regulation of pre-implantation embryo development
dc.relationSFRH-BD/90463/2012pt_PT
dc.relationNot Available
dc.relation.publisherversionhttps://bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-020-00216-2pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBlastocystpt_PT
dc.subjectDevelopmentpt_PT
dc.subjectHatchingpt_PT
dc.subjectNotchpt_PT
dc.subjectMousept_PT
dc.titleNotch signaling in mouse blastocyst development and hatchingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNotch-Wnt signaling cross-talk in the regulation of pre-implantation embryo development
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FCVT-REP%2F2289%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1438%2FCT0001/PT
oaire.citation.conferencePlaceLondon, Englandpt_PT
oaire.citation.titleBMC Developmental Biologypt_PT
oaire.citation.volume20(1):9pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamDL 57/2016
person.familyNamede Moura Carita Dinis Murta
person.familyNameLopes-da-Costa
person.givenNameDaniel José
person.givenNameLuís
person.identifier.ciencia-id1C16-6AF2-977C
person.identifier.ciencia-id0A1F-AC68-0919
person.identifier.orcid0000-0002-5583-485X
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4798d3c9-ee88-4abb-bf40-62d94ab87719
relation.isAuthorOfPublicatione2277e06-ec5b-4f68-8a39-4e4e27389e9c
relation.isAuthorOfPublication.latestForDiscovery4798d3c9-ee88-4abb-bf40-62d94ab87719
relation.isProjectOfPublication049d00ba-b004-4f84-bce3-cd433251ea40
relation.isProjectOfPublication6150b3b2-9902-42a1-8357-05f374227cae
relation.isProjectOfPublication.latestForDiscovery6150b3b2-9902-42a1-8357-05f374227cae

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