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Expression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomi

2021Journal article Open access
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dc.contributor.authorSerrazina, Susana
dc.contributor.authorMachado, Helena
dc.contributor.authorCosta, Rita Lourenço
dc.contributor.authorDuque, Paula
dc.contributor.authorMalhó, Rui
dc.date.accessioned2021-05-28T10:11:34Z
dc.date.available2021-05-28T10:11:34Z
dc.date.issued2021
dc.descriptionOriginal Researchpt_PT
dc.description.abstractAllene oxide synthase (AOS) is a key enzyme of the jasmonic acid (JA) signaling pathway. The AOS gene was previously found to be upregulated in an Asian chestnut species resistant to infection by the oomycete Phytophthora cinnamomi (Castanea crenata), while lower expression values were detected in the susceptible European chestnut (Castanea sativa). Here, we report a genetic and functional characterization of the C. crenata AOS (CcAOS) upon its heterologous gene expression in a susceptible ecotype of Arabidopsis thaliana, which contains a single AOS gene. It was found that Arabidopsis plants expressing CcAOS delay pathogen progression and exhibit more vigorous growth in its presence. They also show upregulation of jasmonic acid and salicylic acid-related genes. As in its native species, heterologous CcAOS localized to plastids, as revealed by confocal imaging of the CcAOS-eGFP fusion protein in transgenic Arabidopsis roots. This observation was confirmed upon transient expression in Nicotiana benthamiana leaf epidermal cells. To further confirm a specific role of CcAOS in the defense mechanism against the pathogen, we performed crosses between transgenic CcAOS plants and an infertile Arabidopsis AOS knockout mutant line. It was found that plants expressing CcAOS exhibit normal growth, remain infertile but are significantly more tolerant to the pathogen than wild type plants. Together, our results indicate that CcAOS is an important player in plant defense responses against oomycete infection and that its expression in susceptible varieties may be a valuable tool to mitigate biotic stress responsespt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSerrazina S, Machado H, Costa RL, Duque P and Malhó R (2021) Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi. Front. Plant Sci. 12:628697pt_PT
dc.identifier.doi10.3389/fpls.2021.628697pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/21375
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers in Plant Sciencept_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectArabidopsis thalianapt_PT
dc.subjectallene oxide synthasept_PT
dc.subjectplant defensept_PT
dc.subjectgene functional analysispt_PT
dc.subjectPhytophthora cinnamomipt_PT
dc.subjectCastanea crenatapt_PT
dc.subjectCastanea sativapt_PT
dc.titleExpression of Castanea crenata allene oxide synthase in Arabidopsis improves defense to Phytophthora cinnamomipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleFrontiers in Plant Sciencept_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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