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Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression

2022-02-26Journal article Open access
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dc.contributor.authorTanoeiro, Luis
dc.contributor.authorOleastro, Mónica
dc.contributor.authorNunes, Alexandra
dc.contributor.authorMarques, Andreia T.
dc.contributor.authorDuarte, Sílvia Vaz
dc.contributor.authorGomes, João Paulo
dc.contributor.authorMatos, António Pedro Alves
dc.contributor.authorVítor, Jorge M. B.
dc.contributor.authorVale, Filipa
dc.date.accessioned2023-08-14T12:02:06Z
dc.date.available2023-08-14T12:02:06Z
dc.date.issued2022-02-26
dc.date.updated2022-10-19T21:27:46Z
dc.description.abstractCampylobacter coli and C. jejuni, the causing agents of campylobacteriosis, are described to be undergoing introgression events, i.e., the transference of genetic material between different species, with some isolates sharing almost a quarter of its genome. The participation of phages in introgression events and consequent impact on host ecology and evolution remain elusive. Three distinct prophages, named C. jejuni integrated elements 1, 2, and 4 (CJIE1, CJIE2, and CJIE4), are described in C. jejuni. Here, we identified two unreported prophages, Campylobacter coli integrated elements 1 and 2 (CCIE1 and CCIE2 prophages), which are C. coli homologues of CJIE1 and CJIE2, respectively. No induction was achieved for both prophages. Conversely, induction assays on CJIE1 and CJIE2 point towards the inducibility of these prophages. CCIE2-, CJIE1-, and CJIE4-like prophages were identified in a Campylobacter spp. population of 840 genomes, and phylogenetic analysis revealed clustering in three major groups: CJIE1-CCIE1, CJIE2-CCIE2, and CJIE4, clearly segregating prophages from C. jejuni and C. coli, but not from human- and nonhuman-derived isolates, corroborating the flowing between animals and humans in the agricultural context. Punctual bacteriophage host-jumps were observed in the context of C. jejuni and C. coli, and although random chance cannot be fully discarded, these observations seem to implicate prophages in evolutionary introgression events that are modulating the hybridization of C. jejuni and C. coli species.pt_PT
dc.description.sponsorshipF.F.V. is funded by Fundação para a Ciência e a Tecnologia (FCT) through an Assistant Researcher grant CEECIND/03023/2017, and a project grant (PTDC/BTM-SAL/28978/2017) that supported this work. The work is partially supported by Na-tional funds from FCT, projects UIDB/04138/2020 and UIDP/04138/2020. Campylobacter strains were sequenced under the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020—Operational Programme for Competitiveness and Internationalization (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Oper-ational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by FCT. This work was also supported by Fundos FEDER through the Programa Operacional Factores de Competitivida-de—COMPETE and by Fundos Nacionais through the FCT within the scope of the project UID/BIM/00009/2019 (Centre for Toxicogenomics and Human Health-ToxOmics).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTanoeiro L, Oleastro M, Nunes A, Marques AT, Duarte SV, Gomes JP, et al. Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression. Microorganisms [Internet]. 2022 Feb 26;10(3):516. Available from: http://dx.doi.org/10.3390/microorganisms10030516pt_PT
dc.identifier.doi10.3390/microorganisms10030516pt_PT
dc.identifier.issn2076-2607
dc.identifier.slugcv-prod-2788847
dc.identifier.urihttp://hdl.handle.net/10451/58866
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPOCI-01-0145-FEDER-022184pt_PT
dc.relationNot Available
dc.relationPhage-Enzybiotic: dealing with critical pathogenic antibiotic-resistant bacteria
dc.relationResearch Institute for Medicines
dc.relationResearch Institute for Medicines
dc.relationCentre for Toxicogenomics and Human Health
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/10/3/516pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBacteriophagept_PT
dc.subjectIntrogressionpt_PT
dc.subjectCampylobacterpt_PT
dc.subjectHost rangept_PT
dc.titleCryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgressionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNot Available
oaire.awardTitlePhage-Enzybiotic: dealing with critical pathogenic antibiotic-resistant bacteria
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03023%2F2017%2FCP1476%2FCT0004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-SAL%2F28978%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIM%2F00009%2F2019/PT
oaire.citation.issue3pt_PT
oaire.citation.startPage516pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStreamCEEC IND 2017
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGodinho Tanoeiro
person.familyNameOleastro
person.familyNameMarques
person.familyNameGomes
person.familyNameVítor
person.familyNameVale
person.givenNameLuis
person.givenNameMónica
person.givenNameAndreia
person.givenNameJoão Paulo
person.givenNameJorge
person.givenNameFilipa
person.identifier1646488
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person.identifier.ciencia-idDC17-69DB-8BB9
person.identifier.ciencia-id6212-831B-E115
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person.identifier.orcid0000-0001-6360-2576
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person.identifier.orcid0000-0002-2697-2399
person.identifier.orcid0000-0001-6486-3444
person.identifier.orcid0000-0003-4635-0105
person.identifier.ridM-3279-2013
person.identifier.ridC-3570-2013
person.identifier.scopus-author-id56799867000
person.identifier.scopus-author-id7801493621
person.identifier.scopus-author-id17136133700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaidFF19-99F4-3964 | Jorge Manuel Barreto Vítor
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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