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Occurrence and molecular characterization of Cryptosporidium spp. in mammals and reptiles at the Lisbon Zoo

dc.contributor.authorAlves, Margarida
dc.contributor.authorXiao, Lihua
dc.contributor.authorLemos, Vanessa
dc.contributor.authorZhou, Ling
dc.contributor.authorCama, Vitaliano
dc.contributor.authorBarão da Cunha, Margarida
dc.contributor.authorMatos, Olga
dc.contributor.authorAntunes, Francisco
dc.date.accessioned2025-07-30T11:40:35Z
dc.date.available2025-07-30T11:40:35Z
dc.date.issued2005
dc.description© Springer-Verlag 2005pt_PT
dc.description.abstractThe presence of Cryptosporidium parasites in mammals and reptiles kept at the Lisbon Zoo was investigated. A total of 274 stool samples were collected from 100 mammals and 29 reptiles. The species and genotype of the isolates identified by light microscopy were determined by nested PCR and sequence analysis of a fragment of the small subunit rRNA gene. Cryptosporidium oocysts were found in one black wildebeest (Connochaetes gnou), one Prairie bison (Bison bison bison) and in one Indian star tortoise (Geochelone elegans). The PCR and sequence analysis of these three isolates showed that those excreted by the Prairie bison were Cryptosporidium mouse genotype, those from the black wildebeest were from a new Cryptosporidium genotype and those infecting the Indian star tortoise were Cryptosporidium tortoise genotype. The present work reports a new Cryptosporidium genotype in a black wildebeest and the first finding of the Cryptosporidium mouse genotype in a ruminant.pt_PT
dc.description.sponsorshipThis work was supported by the projects POCTI/ESP/43635/99 and POCTI/ESP/46369/2002 from the Fundação para a Ciência e Tecnologia (FCT). Margarida Alves received a PhD thesis grant from FCT (SFRH/BD/2898/200).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationParasitol Res. 2005 Sep;97(2):108-112pt_PT
dc.identifier.doi10.1007/s00436-005-1384-9pt_PT
dc.identifier.eissn1432-1955
dc.identifier.issn0932-0113
dc.identifier.urihttp://hdl.handle.net/10400.5/102543
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationPOCTI/ESP/43635/99pt_PT
dc.relationStudy of the Transmission Dynamics of Cryptosporidium Using Molecular Analysis of isolates from Humans, Animals and Water
dc.relation.publisherversionhttps://link.springer.com/journal/436pt_PT
dc.subjectCryptosporidiosispt_PT
dc.subjectCryptosporidium Oocystpt_PT
dc.subjectCryptosporidium infectionpt_PT
dc.subjectMouse genotypept_PT
dc.subjectBison Bisonpt_PT
dc.titleOccurrence and molecular characterization of Cryptosporidium spp. in mammals and reptiles at the Lisbon Zoopt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStudy of the Transmission Dynamics of Cryptosporidium Using Molecular Analysis of isolates from Humans, Animals and Water
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FESP%2F46369%2F2002/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F2898%2F2000/PT
oaire.citation.endPage112pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage108pt_PT
oaire.citation.titleParasitology Researchpt_PT
oaire.citation.volume97pt_PT
oaire.fundingStreamPOCI
person.familyNameAntunes
person.givenNameFrancisco
person.identifier.orcid0000-0001-7932-1154
person.identifier.scopus-author-id7006648941
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa28493a5-9101-4173-8863-74ee946d6ced
relation.isAuthorOfPublication.latestForDiscoverya28493a5-9101-4173-8863-74ee946d6ced
relation.isProjectOfPublication3b0d9986-644c-4bbc-90f1-bfd30cdf050d
relation.isProjectOfPublicationa509f549-0f0b-448f-aef9-cbcebd6dbb43
relation.isProjectOfPublication.latestForDiscovery3b0d9986-644c-4bbc-90f1-bfd30cdf050d

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