Publicação
Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria
| dc.contributor.author | Anand, Paras K. | |
| dc.contributor.author | Anand, Ellis | |
| dc.contributor.author | Bleck, Christopher K. E. | |
| dc.contributor.author | Anes, Elsa | |
| dc.contributor.author | Griffiths, Gareth | |
| dc.date.accessioned | 2012-02-28T16:09:01Z | |
| dc.date.available | 2012-02-28T16:09:01Z | |
| dc.date.issued | 2010-04 | |
| dc.description | © 2010 Anand et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | eng |
| dc.description.abstract | Background: Exosomes are endosome-derived vesicles that are released when multi-vesicular bodies (MVBs) fuse with the plasma membrane. Exosomes released from mycobacteria-infected cells have recently been shown to be pro-inflammatory. A prominent host molecule that is found within these exosomes is Hsp70, a member of the heat-shock family of proteins. Methodology/Principal Findings: We first characterized the exosomes purified from control and mycobacteria-infected cells. We found that relative to uninfected cells, macrophages infected with M. smegmatis and M. avium release more exosomes and the exosomes they released had more Hsp70 on their surface. Both exosomes and exogenous Hsp70 treatment of macrophages led to NF-kB activation and TNFa release in uninfected macrophages; Hsp70 levels were elevated in mycobacteria-infected cells. Macrophage treatment with Hsp70 also led to increase in the phagocytosis and maturation of latex-bead phagosomes. Finally, Hsp70 pre-incubation of M. smegmatis- and M. avium-infected cells led to increased phago-lysosome fusion, as well as more killing of mycobacteria within macrophages. Conclusions/Significance: Our results fit into an emerging concept whereby exosomes-containing Hsp70 are effective inducers of inflammation, also in response to mycobacterial infection. | eng |
| dc.description.sponsorship | E.A. was supported by Fundação para a Ciência e a Tecnologia (FCT) Grant PIC/IC/82859/2007 and PTDC/SAU-MII/098024/2008. P.K.A was supported by a post-doctoral research grant from Alexander von Humboldt foundation, Germany and Marie-Curie fellowship from European Union, FP6 programme MIF1-CT- 2006-039351. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | por |
| dc.identifier.citation | PLoS ONE | April 2010 | Volume 5 | Issue 4 | e10136 | por |
| dc.identifier.issn | 1932-6203 | |
| dc.identifier.uri | http://hdl.handle.net/10451/5453 | |
| dc.identifier.uri | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010136 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Public Library of Science | por |
| dc.subject | Exosomes | por |
| dc.subject | Hsp70 | por |
| dc.subject | Inflammation | por |
| dc.subject | Mycobacteria | por |
| dc.subject | Mycobacterial infection | por |
| dc.title | Exosomal Hsp70 induces a pro-inflammatory response to foreign particles including mycobacteria | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.title | PLoS ONE | por |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |