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Genetic characterization of the dihydrofolate reductase gene of Pneumocystis jirovecii isolates from Portugal

2006Journal article Restricted access
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dc.contributor.authorCosta, Marina C.
dc.contributor.authorEsteves, Francisco
dc.contributor.authorAntunes, Francisco
dc.contributor.authorMatos, Olga
dc.date.accessioned2025-07-31T11:10:30Z
dc.date.available2025-07-31T11:10:30Z
dc.date.issued2006
dc.description© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.pt_PT
dc.description.abstractObjectives: The aim of the present study was to evaluate the genetic variation of Pneumocystis jirovecii dihydrofolate reductase (DHFR) gene in an immunocompromised Portuguese population and to investigate the possible association between DHFR genotypes and P. jirovecii pneumonia (PcP) prophylaxis with co-trimoxazole. Methods: One hundred and thirty-eight P. jirovecii isolates were submitted to DHFR genetic characterization by PCR and sequencing. Results: In the studied population, 72.7% of the patients presented sequences identical to the wild-type sequence of the P. jirovecii DHFR gene and 27.3% presented point substitutions. A total of nine substitution sites were identified; four synonymous substitutions at nucleotide positions 201, 272, 312 and 381 were detected in 31 patients. Five non-synonymous substitutions were observed, leading to the DHFR mutations Leu-13→Ser, Asn-23→Ser, Ser-31→Phe, Met-52→Leu and Ala-67→Val. With the exception of the polymorphism at position 312 and the mutation at codon 52, all polymorphisms were reported in this study for the first time. Conclusions: Our results suggest that DHFR gene polymorphisms are frequent in the Portuguese immunocompromised population but do not seem to be associated with PcP prophylaxis failure (P = 0.748 and P = 0.730).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Antimicrob Chemother. 2006 Dec;58(6):1246-1249pt_PT
dc.identifier.doi10.1093/jac/dkl411pt_PT
dc.identifier.eissn1460-2091
dc.identifier.issn0305-7453
dc.identifier.urihttp://hdl.handle.net/10400.5/102584
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relation.publisherversionhttps://academic.oup.com/jacpt_PT
dc.subjectPolymorphismspt_PT
dc.subjectMutationspt_PT
dc.subjectCo-trimoxazolept_PT
dc.subjectDrug resistancept_PT
dc.titleGenetic characterization of the dihydrofolate reductase gene of Pneumocystis jirovecii isolates from Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1249pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage1246pt_PT
oaire.citation.titleJournal of Antimicrobial Chemotherapypt_PT
oaire.citation.volume58pt_PT
person.familyNameCosta
person.familyNameAntunes
person.familyNameGuerreiro de Matos
person.givenNameMarina
person.givenNameFrancisco
person.givenNameOlga Maria
person.identifier.ciencia-id6818-7810-6CEC
person.identifier.ciencia-id751F-FE84-CCF2
person.identifier.orcid0000-0001-9020-7539
person.identifier.orcid0000-0001-7932-1154
person.identifier.orcid0000-0001-5793-7716
person.identifier.ridO-1710-2014
person.identifier.scopus-author-id55453276400
person.identifier.scopus-author-id7006648941
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc4dcab9e-b873-4587-bb30-5203af150404
relation.isAuthorOfPublicationa28493a5-9101-4173-8863-74ee946d6ced
relation.isAuthorOfPublicationbe64fd9b-8fc7-450b-b84d-c667e7d78760
relation.isAuthorOfPublication.latestForDiscoverybe64fd9b-8fc7-450b-b84d-c667e7d78760

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