| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 1.72 MB | Adobe PDF |
Advisor(s)
Abstract(s)
The effect of seed coating salt-stressed tomato with the bacterium Achromobacter xylosoxidans
BOA4 and/or irrigation with an extract of the marine algae Enteromorpha intestinalis (EI) is herein
evaluated. The plant shoots and roots were harvested separately on day 50, following extensive
saline stress. The addition of BOA4 and/or EI extract resulted in an average increase of 33% in
plant shoot DW, but an averaged decrease of 44% in the root to shoot biomass ratio. Anthocyanin
content increased by over 34% and 44% with EI and BOA4 plus EI treatments, respectively. Since
enhanced protein tyrosine nitration (PTN) is a known plant response to salt stress, the PTN level was
inspected through 3-nitrotyrosine content determination. This was drastically increased by salt stress;
however, BOA4, EI or both caused an averaged PTN decrease of 30% in stressed roots or shoots.
This PTN response could be associated with tomato phenotypic characteristics and is postulated
to be inversely correlated to cytokinin contents in stressed plants, namely cis-zeatin-type-cis-zeatin
(cZ) plus cis-zeatin riboside (cZR), and isopentenyladenine (iP). The latter showed a drastic average
increase by 3.6-fold following BOA4 and/or EI treatments of salinized tomato. This increment could
be related to cytokinin biosynthesis induced by the applied bio-stimulants; IP and derivatives are
the main cytokinins in seaweeds, and Achromobacter xylosoxidans BOA4 was shown to produce up to
17.5 pmol mL−1 of isopentenyladenine. This work is the first report on the influence of bio-stimulants,
used to improve salt stress tolerance, on plant PTN levels; BOA4 and/or EI treatments decreased
PTN, while increasing cis-zeatin-type and iP cytokinins in tomato, the latter showed an enhanced
tolerance to salt stress.
Description
Keywords
Pedagogical Context
Citation
Santana, M.M.; Rosa, A.P.; Zamarreño, A.M.; García-Mina, J.M.; Rai, A.; Cruz, C. Achromobacter xylosoxidans and Enteromorpha intestinalis Extract Improve Tomato Growth under Salt Stress. Agronomy 2022, 12, 934. https://doi.org/10.3390/agronomy12040934
Publisher
MDPI