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Expansion of circulating Foxp3+D25bright CD4+ T cells during specific venom immunotherapy

2007Journal article Unknown
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dc.contributor.authorPereira-Santos, M. C.
dc.contributor.authorBaptista, A. P.
dc.contributor.authorMelo, A.
dc.contributor.authorAlves, R. R.
dc.contributor.authorSoares, R. S.
dc.contributor.authorPedro, E.
dc.contributor.authorPereira-Barbosa, M.
dc.contributor.authorVictorino, R. M. M.
dc.contributor.authorSousa, A. E.
dc.date.accessioned2014-07-14T14:34:29Z
dc.date.available2014-07-14T14:34:29Z
dc.date.issued2007
dc.descriptionJournal compilation © 2007 Blackwell Publishing Ltd, Clinical and Experimental Allergy © 2007 The Authorseng
dc.description.abstractBACKGROUND: Venom immunotherapy (VIT) induces long-lasting immune tolerance to hymenoptera venom antigens, but the underlying mechanisms are not yet clarified. Regulatory T cells are thought to play an important role in allergic diseases and tolerance induction during specific immunotherapy. AIM: Characterize longitudinally the impact of VIT on the pool of circulating regulatory T cells. METHODS: Fourteen hymenoptera venom-allergic patients with severe reactions (grades III-IV) were studied before, 6 and 12 months after starting ultra-rush VIT. Freshly isolated peripheral blood mononuclear cells were surface stained with a panel of markers of T cell differentiation and intracellularly for CTLA-4 and Foxp3 and analysed by flow cytometry. foxp3 mRNA was quantified by real-time PCR. VIT responses were assessed by measuring specific IgG4 and IgE levels. Eleven individuals with no history of insect venom allergy were studied as controls. RESULTS: VIT induces a significant progressive increase in both the proportion and the absolute numbers of regulatory T cells defined as CD25bright and/or Foxp3+ CD4+ T cells. These changes are not related to alterations in the expression of activation markers or imbalances in the naïve/memory T cell compartments. foxp3 mRNA levels also increased significantly during VIT. Of note, the increase in circulating regulatory T cell counts significantly correlates with the venom-specific IgG4/IgE ratio shift. CONCLUSION: VIT is associated with a progressive expansion of circulating regulatory T cells, supporting a role for these cells in tolerance induction.eng
dc.description.sponsorshipAPB and RSS received scholarships from GlaxoSmithKline and Fundação para a Ciência e Tecnologia, Portugal, respectively.eng
dc.identifier.citationClinical and Experimental Allergy, 38, 291–297eng
dc.identifier.doihttp://dx.doi.org/10.1111/j.1365-2222.2007.02887.x
dc.identifier.issn0954-7894
dc.identifier.urihttp://hdl.handle.net/10451/11465
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherBlackwell Publishingeng
dc.subjectCD25brighteng
dc.subjectFoxp3eng
dc.subjectRegulatory T cellseng
dc.subjectSpecific immunotherapyeng
dc.subjectVenom allergyeng
dc.titleExpansion of circulating Foxp3+D25bright CD4+ T cells during specific venom immunotherapyeng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleClinical and Experimental Allergyeng
rcaap.rightsclosedAccesspor
rcaap.typearticlepor

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