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Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM

2019-03-29Artigo científico Acesso aberto
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dc.contributor.authorPinho, MD
dc.contributor.authorFoster, G
dc.contributor.authorPomba, C.
dc.contributor.authorMachado, MP
dc.contributor.authorBaily, JL
dc.contributor.authorKuiken, T
dc.contributor.authorMelo-Cristino, J
dc.contributor.authorRamirez, M
dc.contributor.authorVaz, T
dc.contributor.authorGiao, M
dc.date.accessioned2021-10-13T23:04:47Z
dc.date.available2021-10-13T23:04:47Z
dc.date.issued2019-03-29
dc.descriptionResearch Areas: Microbiologypt_PT
dc.description.abstractABSTRACT - Streptococcus canis is an animal pathogen which occasionally causes infections in humans. The S. canis M-like protein (SCM) encoded by the scm gene, is its best characterized virulence factor but previous studies suggested it could be absent in a substantial fraction of isolates. We studied the distribution and variability of the scm gene in 188 S. canis isolates recovered from companion animals (n = 152), wild animal species (n = 20), and humans (n = 14). Multilocus sequence typing, including the first characterization of wildlife isolates, showed that the same lineages are present in all animal hosts, raising the possibility of extensive circulation between species. Whole-genome analysis revealed that emm-like genes found previously in S. canis correspond to divergent scm genes, indicating that what was previously believed to correspond to two genes is in fact the same scm locus. We designed primers allowing for the first time the successful amplification of the scm gene in all isolates. Analysis of the scm sequences identified 12 distinct types, which could be divided into two clusters: group I (76%, n = 142) and group II (24%, n = 46) sharing little sequence similarity. The predicted group I SCM showed extensive similarity with each other outside of the N-terminal hypervariable region and a conserved IgG binding domain. This domain was absent from group II SCM variants found in isolates previously thought to lack the scm gene, which also showed greater amino acid variability. Further studies are necessary to elucidate the possible host interacting partners of the group II SCM variants and their role in virulence.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPinho MD, Foster G, Pomba C, Machado MP, Baily JL, Kuiken T, Melo-Cristino J, Ramirez M et al. 2019. Streptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCM. Frontiers in Microbiology 10:631pt_PT
dc.identifier.doi10.3389/fmicb.2019.00631pt_PT
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10400.5/22242
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.relationLISBOA-01-0145-FEDER-016417pt_PT
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmicb.2019.00631/fullpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectStreptococcus canispt_PT
dc.subjectmultilocus sequence typingpt_PT
dc.subjectM-like protein (SCM) genept_PT
dc.subjectwildlifept_PT
dc.subjectgenomept_PT
dc.titleStreptococcus canis are a single population infecting multiple animal hosts despite the diversity of the universally present M-Like Protein SCMpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/154779/PT
oaire.citation.conferencePlaceUniversity of Texas MD Anderson Cancer Center, United Statespt_PT
oaire.citation.issue631pt_PT
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volumev10pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMatias Ferreira Pomba
person.givenNameMaria Constança
person.identifier.ciencia-idDA1D-20A8-7F3C
person.identifier.orcid0000-0002-0504-6820
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication53f8cdd6-0030-47fd-9881-a78557579f70
relation.isAuthorOfPublication.latestForDiscovery53f8cdd6-0030-47fd-9881-a78557579f70
relation.isProjectOfPublication90707069-d0b0-4d72-a7b1-02aa93ac23f7
relation.isProjectOfPublication.latestForDiscovery90707069-d0b0-4d72-a7b1-02aa93ac23f7

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