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Lymphocyte reactivity to ex-vivo drug antigens in drug-induced hepatitis

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dc.contributor.authorMaria, Vasco
dc.contributor.authorPinto, Ligia
dc.contributor.authorVictorino, Rui
dc.date.accessioned2022-03-30T15:52:55Z
dc.date.available2022-03-30T15:52:55Z
dc.date.issued1994
dc.descriptionCopyright © Journal of Hepatology 1994pt_PT
dc.description.abstractThe diagnosis of drug-induced hepatitis is usually based on clinical criteria, with emphasis on both the temporal relationship between drug intake and liver injury and the exclusion of alternative causes. In vitro tests of lymphocyte sensitization to drugs are considered to have a low sensitivity. We investigated the possibility of detecting lymphocyte reactivity to drugs in drug-induced hepatitis by analyzing the lymphocyte proliferative responses to ex-vivo drug or metabolite antigens to improve the sensitivity of the in vitro test. Lymphocyte proliferative responses to five different concentrations of the drug and to ex-vivo drug antigens (serum collected from normal subjects after the ingestion of the drugs) were analyzed in 25 patients with a clinical diagnosis of drug-induced hepatitis, 27 healthy subjects and 10 individuals with a recent exposure to the same drugs without development of adverse drug reactions. In seven of the 25 patients, lymphocyte reactivity to drugs was detected (28%). The use of sera collected from healthy volunteers after drug intake (ex-vivo drug antigens) and the addition of a prostaglandin inhibitor to the cultures allowed the detection of lymphocyte sensitization in seven additional cases, increasing the detection ability from 28% to 56%. We suggest that the use of ex-vivo drug antigens may represent a significant contribution to the identification of the drug involved in cases of drug-induced hepatitis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Hepatol. 1994 Aug;21(2):151-158pt_PT
dc.identifier.doi10.1016/S0168-8278(05)80388-5pt_PT
dc.identifier.eissn1600-0641
dc.identifier.issn0168-8278
dc.identifier.urihttp://hdl.handle.net/10451/52107
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/journal-of-hepatologypt_PT
dc.subjectDiagnosispt_PT
dc.subjectDrug metabolitespt_PT
dc.subjectImmunological testspt_PT
dc.subjectLymphocyte proliferationpt_PT
dc.titleLymphocyte reactivity to ex-vivo drug antigens in drug-induced hepatitispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage158pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage151pt_PT
oaire.citation.titleJournal of Hepatologypt_PT
oaire.citation.volume21pt_PT
person.familyNameMaria
person.familyNameVictorino
person.givenNameVasco
person.givenNameRui
person.identifier.orcid0000-0003-4378-0265
person.identifier.orcid0000-0001-7567-1704
person.identifier.ridN-7948-2013
person.identifier.scopus-author-id7004664870
person.identifier.scopus-author-id7005391596
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication559428fd-8556-4a3c-9039-f39c68210715
relation.isAuthorOfPublicationa37b91c2-38bf-4d67-a83f-8687995135ab
relation.isAuthorOfPublication.latestForDiscovery559428fd-8556-4a3c-9039-f39c68210715

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