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Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials

2020-08-05Journal article Open access
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dc.contributor.authorLopes, Joana
dc.contributor.authorCoelho, João Miguel Pinto
dc.contributor.authorVieira, Pedro Manuel Cardoso
dc.contributor.authorViana, Ana Silveira
dc.contributor.authorGaspar, Maria Manuela
dc.contributor.authorReis, Catarina Pinto
dc.date.accessioned2022-04-12T12:33:24Z
dc.date.available2022-04-12T12:33:24Z
dc.date.issued2020-08-05
dc.date.updated2022-02-22T10:53:55Z
dc.descriptionSupplementary Materials - The following are available online at https://www.mdpi.com/2079-4991/10/8/1536/s1, Video S1: Thermal activation of HAOA-coated GNPs.pt_PT
dc.description.abstractCancer like melanoma is a complex disease, for which standard therapies have significant adverse side effects that in most cases are ineffective and highly unspecific. Thus, a new paradigm has come with the need of achieving alternative (less invasive) and effective therapies. In this work, biocompatible gold nanoparticles (GNPs) coated with hyaluronic acid and oleic acid were prepared and characterized in terms of size, morphology and cytotoxicity in the presence of Saccharomyces cerevisiae, and two cell lines, the keratinocytes (healthy skin cells, HaCat) and the melanoma cells (B16F10). Results showed that these GNPs absorb within the near-infrared region (750–1400 nm), in the optical therapeutic window (from 650 to 1300 nm), in contrast to other commercial gold nanoparticles, which enables light to penetrate into deep skin layers. A laser emitting in this region was applied and its effect also analyzed. The coated GNPs showed a spherical morphology with a mean size of 297 nm without cytotoxic effects towards yeast and tested cell lines. Nevertheless, after laser irradiation, a reduction of 20% in B16F10 cell line viability was observed. In summary, this work appears to be a promising strategy for the treatment of non-metastatic melanoma or other superficial tumors.pt_PT
dc.description.sponsorshipThis research was funded by Fundação para a Ciência e a Tecnologia (FCT) through the projects: UIDB/00645/2020, UID/DTP/04138/2019, PTDC/MED-QUI/31721/2017 as well as by the PhD fellowship SFRH/BD/148044/2019 (J.L.).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLopes J, Coelho JMP, Vieira PMC, Viana AS, Gaspar MM, Reis C. Preliminary assays towards melanoma cells using phototherapy with gold-based nanomaterials. Nanomaterials [Internet]. 2020;10(8):1536. Disponível em: https://www.mdpi.com/2079-4991/10/8/1536pt_PT
dc.identifier.doihttps://doi.org/10.3390/nano10081536pt_PT
dc.identifier.issn2079-4991
dc.identifier.slugcv-prod-2446145
dc.identifier.urihttp://hdl.handle.net/10451/52298
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationInstitute of <biophysics and Biomedical Engineering
dc.relationResearch Institute for Medicines
dc.relationNANOFORMULATED HYBRID MOLECULES FOR SPECIFIC TAREGETING MELANOMA METASTASIS
dc.relationTargeting superficial tumors using a nanotechnological hybrid approach
dc.relation.publisherversionhttps://www.mdpi.com/2079-4991/10/8/1536pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectmelanomapt_PT
dc.subjectgold nanoparticlespt_PT
dc.subjectbioproductionpt_PT
dc.subjectlaser photothermal therapypt_PT
dc.subjectin vitro modelspt_PT
dc.subjectexperimental resultspt_PT
dc.titlePreliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterialspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInstitute of <biophysics and Biomedical Engineering
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleNANOFORMULATED HYBRID MOLECULES FOR SPECIFIC TAREGETING MELANOMA METASTASIS
oaire.awardTitleTargeting superficial tumors using a nanotechnological hybrid approach
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00645%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMED-QUI%2F31721%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F148044%2F2019/PT
oaire.citation.issue8pt_PT
oaire.citation.startPage1536pt_PT
oaire.citation.titleNanomaterialspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
person.familyNamede Jesus Guilherme Gaspar
person.familyNameBeco Pinto Reis
person.givenNameMaria Manuela
person.givenNameAna Catarina
person.identifierC-2024-2014
person.identifierM-8132-2013
person.identifier.ciencia-id3A10-0732-13F5
person.identifier.ciencia-id1317-A7D6-3B1C
person.identifier.orcid0000-0001-6814-7226
person.identifier.orcid0000-0002-1046-4031
person.identifier.scopus-author-id15834627800
person.identifier.scopus-author-id14027161300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid201A-EC2A-8CD7 | Joana Margarida Delgado Lopes
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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