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Cloning, Characterization, and Expression Levels of the Nectin Gene from the Tube Feet of the Sea Urchin Paracentrotus Lividus

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dc.contributor.authorToubarro, Duarte
dc.contributor.authorGouveia, Analuce
dc.contributor.authorRibeiro, Raquel Mesquita
dc.contributor.authorSimões, Nélson
dc.contributor.authorda Costa, Gonçalo
dc.contributor.authorCordeiro, Carlos
dc.contributor.authorSantos, Romana
dc.date.accessioned2020-01-19T18:20:55Z
dc.date.available2020-01-19T18:20:55Z
dc.date.issued2016
dc.description.abstractMarine bioadhesives perform in ways that manmade products simply cannot match, especially in wet environments. Despite their technological potential, bioadhesive molecular mechanisms are still largely understudied, and sea urchin adhesion is no exception. These animals inhabit wave-swept shores, relying on specialized adhesive organs, tube feet, composed by an adhesive disc and a motile stem. The disc encloses a duo-gland adhesive system, producing adhesive and deadhesive secretions for strong reversible substratum attachment. The disclosure of sea urchin Paracentrotus lividus tube foot disc proteome led to the identification of a secreted adhesion protein, Nectin, never before reported in adult adhesive organs but, that given its adhesive function in eggs/embryos, was pointed out as a putative substratum adhesive protein in adults. To further understand Nectin involvement in sea urchin adhesion, Nectin cDNA was amplified for the first time from P. lividus adhesive organs, showing that not only the known Nectin mRNA, called Nectin-1 (GenBank AJ578435), is expressed in the adults tube feet but also a new mRNA sequence, called Nectin-2 (GenBank KT351732), differing in 15 missense nucleotide substitutions. Nectin genomic DNA was also obtained for the first time, indicating that both Nectin-1 and Nectin-2 derive from a single gene. In addition, expression analysis showed that both Nectins are overexpressed in tube feet discs, its expression being significantly higher in tube feet discs from sea urchins just after collection from the field relative to sea urchin from aquarium. These data further advocate for Nectin involvement in sea urchin reversible adhesion, suggesting that its expression might be regulated according to the hydrodynamic conditions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s10126-016-9698-4pt_PT
dc.identifier.issn1436-2228
dc.identifier.urihttp://hdl.handle.net/10451/41067
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s10126-016-9698-4pt_PT
dc.subjectMarine adhesionpt_PT
dc.subjectSea urchinpt_PT
dc.subjectTube feetpt_PT
dc.subjectSubstratum adhesive proteinpt_PT
dc.subjectNectinpt_PT
dc.titleCloning, Characterization, and Expression Levels of the Nectin Gene from the Tube Feet of the Sea Urchin Paracentrotus Lividuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage383pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage372pt_PT
oaire.citation.titleMarine Biotechnologypt_PT
oaire.citation.volume18pt_PT
person.familyNameLopes Almeida dos Santos
person.givenNameRomana
person.identifier.ciencia-idBD12-1D9C-3627
person.identifier.orcid0000-0001-7720-6806
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd7b58331-4fdc-410b-878b-199a8f2975cb
relation.isAuthorOfPublication.latestForDiscoveryd7b58331-4fdc-410b-878b-199a8f2975cb

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