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ACOI 58 and ACOI 1257, two different Portuguese strains of colonial green microalga Botryococcus
bruanii Kutz obtained from Coimbra Collection of Algae (ACOI), were evaluated on their potential for
biofuel production, which was assessed from batch culture in photobioreactors under indoors and
outdoors conditions using pretreated waste water and semisynthetic culture media. In the experiments,
the maximum specific growth rate achieved was 0.4 day-1 with doubling times ranging from 2 to 7 days
in the fastest growing phase. However, both strains showed ability to absorb nutrients in waste water
cultures, to grow and accumulate oil. The maximum lipid extraction on a dry matter basis was 36% in
CHU media and 29% in waste water cultures, which revealed the promising potential of these B. braunii
isolates to be used in biofuel production applications. This is also supported by the predominance of
oleic (C18:1, 42 to 63%) and palmitic acid (C16:0, 8 to 18%) in the lipid extract, since the methyl esters of
these fatty acids (FAME) are ideal for biodiesel production. In summary, although these strains of B.
braunii have presented a typical growth for this species, they produced considerable lipid content and
were able to grow in waste water and under outdoors conditions that warrants further investment in
their study.
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Microalga Botryococcus braunii wastewater photobioreactors biofuels
