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Skeletal muscle metabolomics and blood biochemistry analysis reveal metabolic changes associated with dietary amino acid supplementation in dairy calves
Orientador(es)
Resumo(s)
The effects of different amino acid (AA) supplementations of milk protein-based milk replacers
in pre-ruminant calves from 3 days to 7 weeks of age were studied. Animals were divided into 4
groups: Ctrl) Control group fed with milk protein-based milk replacer without supplementation; GP)
supplementation with 0.1% glycine and 0.3% proline; FY) supplementation with 0.2% phenylalanine
and 0.2% tyrosine; MKT) supplementation with 0.62% lysine, 0.22% methionine and 0.61% threonine.
For statistical analysis, t-test was used to compare AA-supplemented animals to the Ctrl group. At week
7, body weight and average daily gain (ADG) were measured and blood samples and skeletal muscle
biopsies were taken. Blood biochemistry analytes related to energy metabolism were determined
and it was shown that MKT group had higher serum creatinine and higher plasma concentration
of three supplemented AAs as well as arginine compared with the Ctrl group. GP group had similar
glycine/proline plasma concentration compared with the other groups while in FY group only plasma
phenylalanine concentration was higher compared with Control. Although the AA supplementations
in the GP and FY groups did not affect average daily gain and metabolic health profile from serum, the
metabolome analysis from skeletal muscle biopsy revealed several differences between the GP-FY
groups and the Ctrl-MKT groups, suggesting a metabolic adaptation especially in GP and FY groups
Descrição
Palavras-chave
dairy calves amino acid supplementation muscle blood biochemistry
Contexto Educativo
Citação
ScIentific Reports , (2018) 8:13850
Editora
Nature Publishing Group