Repository logo
 
Publication

Impact of γ’γ’ fibrinogen interaction with red blood cells on fibrin clots

2018Journal article Restricted access
Simple item page
dc.contributor.authorGuedes, Ana Filipa
dc.contributor.authorCarvalho, Filomena Almeida
dc.contributor.authorDomingues, Marco
dc.contributor.authorMacrae, Fraser L.
dc.contributor.authorMcPherson, Helen R.
dc.contributor.authorSabban, Aliaa
dc.contributor.authorMartins, Ivo C.
dc.contributor.authorDuval, Cédric
dc.contributor.authorSantos, Nuno C.
dc.contributor.authorAriёns, Robert A.S.
dc.date.accessioned2019-04-05T11:10:41Z
dc.date.available2019-04-05T11:10:41Z
dc.date.issued2018
dc.description© 2018 Future Medicine Ltd.pt_PT
dc.description.abstractAIM: γ' fibrinogen has been associated with thrombosis. Here the interactions between γ'γ' or γAγA fibrinogen and red blood cells (RBCs), and their role on fibrin clot properties were studied. MATERIALS & METHODS: Atomic Force microscopy (AFM)-based force spectroscopy, rheological, electron and confocal microscopy, and computational approaches were conducted for both fibrinogen variants. RESULTS & CONCLUSION: AFM shows that the recombinant human (rh)γ'γ' fibrinogen increases the binding force and the frequency of the binding to RBCs compared with rhγAγA, promoting cell aggregation. Structural changes in rhγ'γ' fibrin clots, displaying a nonuniform fibrin network were shown by microscopy approaches. The presence of RBCs decreases the fibrinolysis rate and increases viscosity of rhγ'γ' fibrin clots. The full length of the γ' chain structure, revealed by computational analysis, occupies a much wider surface and is more flexible, allowing an increase of the binding between γ' fibers, and eventually with RBCs.pt_PT
dc.description.sponsorshipThis work was funded by Fundação para a Ciência e a Tecnologia–Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal) project PTDC/BBB-BMD/6307/2014 and Fundação Calouste Gulbenkian (Portugal). This work was also supported by LISBOA 01-0145-FEDER-007391 project, cofunded by FEDER, through POR Lisboa 2020 - Programa Operacional Regional de Lisboa, PORTUGAL 2020, and FCT-MCTES. AF Guedes also acknowledges FCT-MCTES PhD fellowship SFRH/BD/84414/2012, IC Martins acknowledges FCT-MCTES Program “Investigador FCT” (IF/00772/2013 Research Contract) and MM Domingues acknowledges FCT-MCTES post-doctoral fellowship SFRH/BPD/122779/2016. RAS is supported by the British Heart Foundation (RG/13/3/30104).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNanomedicine (Lond.) (2018) 13(19), 2491–2505pt_PT
dc.identifier.doi10.2217/nnm-2018-0136pt_PT
dc.identifier.issn1743-5889
dc.identifier.urihttp://hdl.handle.net/10451/37864
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFuture Medicinept_PT
dc.relationLISBOA 01-0145-FEDER-007391pt_PT
dc.relationMolecular interactions of circulating fibrinogen as a risk factor for cardiovascular and cerebrovascular pathologies
dc.relationIF/00772/2013pt_PT
dc.relationRG/13/3/30104pt_PT
dc.relationDelivery and targeting fibrinolytic agents to blood clots
dc.relation.publisherversionhttps://www.futuremedicine.com/loi/nnmpt_PT
dc.subjectFibrin clotpt_PT
dc.subjectFibrinolysispt_PT
dc.subjectRed blood cellpt_PT
dc.subjectThrombosispt_PT
dc.subjectγ' fibrinogenpt_PT
dc.titleImpact of γ’γ’ fibrinogen interaction with red blood cells on fibrin clotspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMolecular interactions of circulating fibrinogen as a risk factor for cardiovascular and cerebrovascular pathologies
oaire.awardTitleDelivery and targeting fibrinolytic agents to blood clots
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-BMD%2F6307%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F84414%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F122779%2F2016/PT
oaire.citation.endPage2505pt_PT
oaire.citation.issue19pt_PT
oaire.citation.startPage2491pt_PT
oaire.citation.titleNanomedicinept_PT
oaire.citation.volume13pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
oaire.fundingStreamOE
person.familyNameGuedes
person.familyNameCarvalho
person.familyNameDomingues
person.familyNameMartins
person.familyNameSantos
person.givenNameAna Filipa
person.givenNameFilomena
person.givenNameMarco
person.givenNameIvo
person.givenNameNuno
person.identifier.ciencia-id4615-4AEC-8D91
person.identifier.ciencia-idE317-92F8-84C6
person.identifier.ciencia-id8C17-BB8B-E8DB
person.identifier.ciencia-idCD13-5E3A-A3C5
person.identifier.orcid0000-0001-6768-1718
person.identifier.orcid0000-0001-6088-3894
person.identifier.orcid0000-0003-1502-1421
person.identifier.orcid0000-0002-9284-8599
person.identifier.orcid0000-0002-0580-0475
person.identifier.ridM-9202-2013
person.identifier.ridG-2534-2013
person.identifier.ridN-7248-2013
person.identifier.scopus-author-id7103069694
person.identifier.scopus-author-id8628164300
person.identifier.scopus-author-id55940818300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication7855d562-098c-4bdc-8980-c240be6a8d18
relation.isAuthorOfPublication547cb993-880c-4608-b1e0-493ad469eecb
relation.isAuthorOfPublicationce0480de-ac1f-4d55-bf83-19e221bb8768
relation.isAuthorOfPublication93078a2a-308d-4285-8592-0ae20057dc09
relation.isAuthorOfPublication4656d912-5a2d-4c80-8921-2bcba3aa441a
relation.isAuthorOfPublication.latestForDiscovery7855d562-098c-4bdc-8980-c240be6a8d18
relation.isProjectOfPublicationab8a6a0e-d04e-405e-9cf1-e3a92e0d8e06
relation.isProjectOfPublication17349880-8f92-460a-8f4f-1e055f7cce86
relation.isProjectOfPublication3ff3136d-1f26-4ec6-b62b-103d20020a86
relation.isProjectOfPublication.latestForDiscoveryab8a6a0e-d04e-405e-9cf1-e3a92e0d8e06

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Y_fibrinogen.pdf
Size:
6.44 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.2 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

IMM - Artigos em Revistas Internacionais