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Heat wave impacts on the model diatom Phaeodactylum tricornutum: Searching for photochemical and fatty acid biomarkers of thermal stress

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dc.contributor.authorFeijão, Eduardo
dc.contributor.authorGameiro, C.
dc.contributor.authorFranzitta, Marco
dc.contributor.authorDuarte, Bernardo
dc.contributor.authorCaçador, Isabel
dc.contributor.authorCabrita, Maria Teresa
dc.contributor.authorMatos, Ana Rita
dc.date.accessioned2019-06-25T09:42:11Z
dc.date.available2019-06-25T09:42:11Z
dc.date.issued2018
dc.description.abstractGlobal warming is increasing the frequency and intensity of extreme thermal events, with inevitable consequences for marine ecosystems and organisms. Phytoplankton is at the base of marine food webs and diatoms are major contributors to global primary production. Therefore, environmental changes, such as heat, influencing growth, physiology and biochemical composition of diatoms, impact other organisms at higher trophic levels. The model diatom Phaeodactylum tricornutum, particularly rich in the long chain omega-3 eicosapentaenoic acid (EPA), and able to accumulate substantial amounts of storage lipids, has recently been the object of numerous works, regarding fundamental aspects of lipid metabolism and exploring its biotechnological potential for biodiesel and aquaculture purposes. The aim of this study was to use P. tricornutum, growing under controlled conditions, to examine the effects of a heat wave, in order to identify heat stress biomarkers. The photosynthetic and respiratory metabolism was investigated by Chlorophyll a fluorescence and by O2 evolution and discussed in connection with changes observed in the composition of photosynthetic pigments and fatty acids. Phaeodactylum tricornutum cells exposed to 26 °C displayed lower photosynthetic O2 production, but similar respiratory rate, comparing to cells at control temperature (18 °C), which is likely related to the biomass decrease observed under heat stress. Heat wave exposed cells also showed a less efficient PSII, higher energy dissipation and higher chlorophyll a and fucoxanthin concentrations, suggesting a heat-induced amplification of the light energy absorption capacity. Heat wave exposed cells showed lower relative EPA contents and double bond indexes, whereas the parameter inversely related to nutritional value, omega 6/omega 3 ratio, increased. Moreover, the analysis of the fatty acid profiles also suggested that heat exposure negatively impacted thylakoid lipids, in agreement with the decrease observed in photosynthesis. Results obtained highlight the negative impact of heat waves on diatom photosynthesis and nutritional value, as well as on their capacity to oxygenate ocean water. Furthermore, physiological parameters as well as fatty acids and photosynthetic pigments signatures, were identified, that could represent expedite biomarkers of thermal stress in future studies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ecolind.2017.07.058pt_PT
dc.identifier.issn1470-160X
dc.identifier.urihttp://hdl.handle.net/10451/38779
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectEicosapentadienoic acid (EPA)pt_PT
dc.subjectOmega-3 fatty acidspt_PT
dc.subjectPhotosynthesispt_PT
dc.subjectBiomarkerspt_PT
dc.subjectPhaeodactylum tricornutumpt_PT
dc.subjectClimate changept_PT
dc.titleHeat wave impacts on the model diatom Phaeodactylum tricornutum: Searching for photochemical and fatty acid biomarkers of thermal stresspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1037pt_PT
oaire.citation.startPage1026pt_PT
oaire.citation.titleEcological Indicatorspt_PT
oaire.citation.volume95pt_PT
person.familyNameGameiro
person.familyNameDuarte
person.familyNameCaçador
person.givenNameCarla
person.givenNameBernardo
person.givenNameIsabel
person.identifier89449
person.identifier.ciencia-id771B-9D07-AB1A
person.identifier.ciencia-id731E-093F-D4C8
person.identifier.ciencia-id631C-9FFE-CA81
person.identifier.orcid0000-0003-2396-3929
person.identifier.orcid0000-0003-1914-7435
person.identifier.orcid0000-0002-4475-6091
person.identifier.ridB-5462-2012
person.identifier.ridH-2001-2011
person.identifier.ridC-2618-2012
person.identifier.scopus-author-id20734253500
person.identifier.scopus-author-id20734149900
person.identifier.scopus-author-id6602533871
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication39490eaa-c76a-4478-9ff0-844df5fb91ca
relation.isAuthorOfPublicationa72f0f40-c224-49ad-ba21-ebb5f9aab09c
relation.isAuthorOfPublication02ecb390-88b9-4e2f-8690-eba66c3b5311
relation.isAuthorOfPublication.latestForDiscovery02ecb390-88b9-4e2f-8690-eba66c3b5311

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