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Group A Streptococci clones associated with invasive infections and pharyngitis in Portugal present differences in emm types, superantigen gene content and antimicrobial resistance

2012-11-27Artigo científico Acesso aberto
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dc.contributor.authorFriães, Ana
dc.contributor.authorPinto, Francisco R.
dc.contributor.authorSilva-Costa, Catarina
dc.contributor.authorRamirez, Mário
dc.contributor.authorCristino, José Melo
dc.date.accessioned2021-07-26T14:20:09Z
dc.date.available2021-07-26T14:20:09Z
dc.date.issued2012-11-27
dc.description© 2012 Friães et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citedpt_PT
dc.description.abstractBackground: A few lineages of Group A streptococci (GAS) have been associated with a reemergence of severe invasive streptococcal disease in developed countries. However, the majority of the comparisons between invasive and non-invasive GAS isolates have been performed for collections of reduced genetic diversity or relied on limited typing information to distinguish clones. We characterized by several typing methods and compared a collection of 160 isolates recovered from normally sterile sites with 320 isolates associated with pharyngitis and recovered in the same time period in Portugal. Results: Although most of the isolates belonged to clones that were equally prevalent in invasive infections and pharyngitis, we identified markers of invasiveness, namely the emm types 1 and 64, and the presence of the speA and speJ genes. In contrast, emm4, emm75, and the ssa and speL/M genes were significantly associated with pharyngitis. There was a strong agreement between the emm type, the superantigen (SAg) genes and the clusters defined by pulsed-field gel electrophoresis (PFGE) profiling. Therefore, combinations of particular emm types and SAg genes frequently co-occurred in the same PFGE cluster, but there was no synergistic or antagonistic interaction between them in determining invasiveness. Only macrolide-susceptible PFGE clones were significantly associated with invasive infections or pharyngitis, while the clones of resistant isolates sharing all other molecular properties analyzed were equally prevalent in the two groups of isolates. Conclusions: This study confirmed the importance of the widely disseminated emm1-T1-ST28 clone in invasive infections but also identified other clones linked to either invasive infections (emm64-ST164) or pharyngitis (emm4-T4-ST39), which may be more limited in their temporal and geographical spread. Clonal properties like some emm types or SAg genes were associated with disease presentation, highlighting the importance of bacterial genetic factors to the outcome of GAS infections, although other, yet unidentified factors may also play an important role.pt_PT
dc.description.sponsorshipThis work was partially supported by Fundação para a Ciência e Tecnologia, Portugal (PTDC/SAU-ESA/72321/2006), Fundação Calouste Gulbenkian and unrestricted research grant from Glaxo SmithKline.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBMC Microbiol. 2012 Nov 27;12:280.pt_PT
dc.identifier.doi10.1186/1471-2180-12-280pt_PT
dc.identifier.eissn1471-2180
dc.identifier.urihttp://hdl.handle.net/10451/49119
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relation.publisherversionhttps://bmcmicrobiol.biomedcentral.com/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectStreptococcus pyogenespt_PT
dc.subjectStreptococcal M proteinpt_PT
dc.subjectExotoxinspt_PT
dc.subjectPharyngitispt_PT
dc.subjectInvasive infectionpt_PT
dc.titleGroup A Streptococci clones associated with invasive infections and pharyngitis in Portugal present differences in emm types, superantigen gene content and antimicrobial resistancept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPTDC/SAU-ESA/72321/2006
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-ESA%2F72321%2F2006/PT
oaire.citation.titleBMC Microbiologypt_PT
oaire.fundingStream3599-PPCDT
person.familyNameAquino Friães
person.familyNameSilva Costa
person.familyNameRamos de Almeida Ramirez
person.familyNameMelo Cristino
person.givenNameAna Isabel
person.givenNameAna Catarina
person.givenNameMário Nuno
person.givenNameJosé
person.identifierB-4993-2008
person.identifier.ciencia-idB616-F64A-ACEF
person.identifier.ciencia-id6517-D034-BADD
person.identifier.ciencia-id9C1C-F2A2-4226
person.identifier.ciencia-id871E-6AD6-F37C
person.identifier.orcid0000-0002-7567-2405
person.identifier.orcid0000-0001-9681-0574
person.identifier.orcid0000-0002-4084-6233
person.identifier.orcid0000-0001-8643-1722
person.identifier.ridH-3726-2013
person.identifier.scopus-author-id7201568476
person.identifier.scopus-author-id7004053640
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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