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In vivo recruitment of exon junction complex proteins to transcription sites in mammalian cell nuclei

2004-04Artigo científico Acesso restrito
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dc.contributor.authorCustódio, Noélia
dc.contributor.authorCarvalho, Célia
dc.contributor.authorCondado, Inês
dc.contributor.authorAntoniou, Michael
dc.contributor.authorBlencowe, Benjamin J.
dc.contributor.authorCarmo-Fonseca, Maria
dc.date.accessioned2021-05-04T14:44:34Z
dc.date.available2021-05-04T14:44:34Z
dc.date.issued2004-04
dc.descriptionCopyright © 2004 RNA Societypt_PT
dc.description.abstractStudies over the past years indicate that there is extensive coupling between nuclear export of mRNA and pre-mRNA processing. Here, we visualized the distribution of exon junction complex (EJC) proteins and RNA export factors relative to sites of abundant pre-mRNA synthesis in the nucleus. We analyzed both HeLa cells infected with adenovirus and murine erythroleukemia (MEL) cells stably transfected with the human beta-globin gene. Using in situ hybridization and confocal microscopy, we observe accumulation of EJC proteins (REF/Aly, Y14, SRm160, UAP56, RNPS1, and Magoh) and core spliceosome components (U snRNPs) at sites of transcription. This suggests that EJC proteins bind stably to pre-mRNA cotranscriptionally. No concentration of the export factors NXF1/TAP, p15, and Dbp5 was detected on nascent transcripts, arguing that in mammalian cells these proteins bind the mRNA shortly before or after release from the sites of transcription. These results also suggest that binding of EJC proteins to the mRNA is not sufficient to recruit TAP-p15, consistent with recent findings showing that the EJC does not play a crucial role in mRNA export. Contrasting to the results obtained in MEL cells expressing normal human beta-globin transcripts, mutant pre-mRNAs defective in splicing and 3'end processing do not colocalize with SRm160, REF, UAP56, or Sm proteins. This shows that the accumulation of EJC proteins at transcription sites requires efficient processing of the nascent pre-mRNAs, arguing that transcription per se is not sufficient for the stable assembly of the EJC.pt_PT
dc.description.sponsorshipThis study was supported by a grant (POCTI/MGI/36547/00) from Fundação para a Ciência e Tecnologia (FCT), Portugal, and by the European Commission (QLG2-CT-2001-01554).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRNA. 2004 Apr;10(4):622-633pt_PT
dc.identifier.doi10.1261/rna.5258504pt_PT
dc.identifier.eissn1469-9001
dc.identifier.issn1355-8382
dc.identifier.urihttp://hdl.handle.net/10451/47642
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCold Spring Harbor Laboratory Presspt_PT
dc.relationPOCTI/MGI/36547/00pt_PT
dc.relationQLG2-CT-2001-01554pt_PT
dc.relation.publisherversionhttps://rnajournal.cshlp.org/pt_PT
dc.subjectPre-mRNA splicingpt_PT
dc.subjectmRNA exportpt_PT
dc.subjectExon junction complexpt_PT
dc.subjectNXF1/TAPpt_PT
dc.titleIn vivo recruitment of exon junction complex proteins to transcription sites in mammalian cell nucleipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage633pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage622pt_PT
oaire.citation.titleRNApt_PT
oaire.citation.volume10pt_PT
person.familyNameFernandes Custódio
person.familyNameCarvalho
person.familyNameCarmo-Fonseca
person.givenNameNoélia Maria
person.givenNameCélia
person.givenNameMaria
person.identifier.ciencia-id8615-44B3-7645
person.identifier.ciencia-idDD18-667D-59FE
person.identifier.ciencia-idB31F-0435-0753
person.identifier.orcid0000-0001-9230-9870
person.identifier.orcid0000-0002-5980-595X
person.identifier.orcid0000-0002-3402-7143
person.identifier.scopus-author-id6508187568
person.identifier.scopus-author-id7007128195
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb01cecb7-4011-4400-84ef-5e8552ccb3e5
relation.isAuthorOfPublication0bc60872-4f9a-44d0-9bd6-13c82c3298c2
relation.isAuthorOfPublicationf4cae50c-2389-4b6a-8e3a-8fd8e538add0
relation.isAuthorOfPublication.latestForDiscoveryf4cae50c-2389-4b6a-8e3a-8fd8e538add0

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