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Nanoscale characterization of the temporary adhesive of the sea urchin Paracentrotus lividus

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dc.contributor.authorViana, Ana S
dc.contributor.authorAlmeida dos Santos, Romana
dc.date.accessioned2020-01-19T21:06:31Z
dc.date.available2020-01-19T21:06:31Z
dc.date.issued2018
dc.description.abstractBackground: Unlike the thin homogeneous films that are typical for adhesives produced by humans, biological adhesives present complex hierarchical micro- and nanostructures. Most studies on marine adhesives have focused on permanent adhesives, whereas the nanostructures of nonpermanent, temporary or reversible adhesives have only been examined in some organisms such as marine flatworms, barnacle cyprids, freshwater cnidaria and echinoderms such as sea cucumbers and sea stars. In this study, the first nanoscale characterization of sea urchin temporary adhesives was performed using atomic force microscopy (AFM). Results: The adhesive topography was similar under dry and native (seawater) conditions, which was comprised of a honeycomb-like meshwork of aggregated globular nanostructures. In terms of adhesion forces, higher values were obtained in dry conditions, reaching up to 50 nN. Under native conditions, lower adhesive forces were obtained (up to 500 pN) but the adhesive seemed to behave like a functional amyloid, as evidenced by the recorded characteristic sawtooth force-extension curves and positive thioflavin-T labelling. Conclusion: Our results confirm that like other temporary adhesives, the sea urchin adhesive footprint nanostructure consists of a meshwork of entangled globular nanostructures. Under native conditions, the adhesive footprints of the sea urchin behaved like a functional amyloid, suggesting that among its proteinaceous constituents there are most likely proteins with amyloid quaternary structures or rich in β-sheets. These results extend our knowledge on sea urchin adhesive composition and mechanical properties essential for the engineering of biomimetic adhesives.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3762/bjnano.9.212pt_PT
dc.identifier.issn2190-4286
dc.identifier.urihttp://hdl.handle.net/10451/41236
dc.language.isoengpt_PT
dc.publisherBeilstein-Institutpt_PT
dc.relation.publisherversionhttps://www.beilstein-journals.org/bjnano/articles/9/212pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectadhesive footprintpt_PT
dc.subjectatomic force microscopypt_PT
dc.subjectnanomechanical propertiespt_PT
dc.subjectsea urchinpt_PT
dc.subjecttemporary adhesionpt_PT
dc.titleNanoscale characterization of the temporary adhesive of the sea urchin Paracentrotus lividuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2286pt_PT
oaire.citation.startPage2277pt_PT
oaire.citation.titleBeilstein Journal of Nanotechnologypt_PT
oaire.citation.volume9pt_PT
person.familyNameLopes Almeida dos Santos
person.givenNameRomana
person.identifier.ciencia-idBD12-1D9C-3627
person.identifier.orcid0000-0001-7720-6806
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd7b58331-4fdc-410b-878b-199a8f2975cb
relation.isAuthorOfPublication.latestForDiscoveryd7b58331-4fdc-410b-878b-199a8f2975cb

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