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Diagnosis of coccidiosis by Eimeria spp. in free-range chickens using Mini-FLOTAC and McMaster techniques - preliminary results

2021-02Artigo científico Acesso aberto
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dc.contributor.authorLozano, João
dc.contributor.authorAnaya, Adriana
dc.contributor.authorRinaldi, Laura
dc.contributor.authorCringoli, Giuseppe
dc.contributor.authorGomes, Lídia
dc.contributor.authorOliveira, Manuela
dc.contributor.authorPaz-Silva, Adolfo
dc.contributor.authorRebelo, Maria Teresa
dc.contributor.authorMadeira de Carvalho, Luís
dc.date.accessioned2021-09-07T10:48:58Z
dc.date.available2021-09-07T10:48:58Z
dc.date.issued2021-02
dc.description.abstractMini-FLOTAC is emerging as a more sensitive and accurate tool to identify gastrointestinal parasites in faecal samples from domestic animals, in comparison with the McMaster method. However, research regarding its specific application in poultry samples, particularly from free-range chickens, is scarce. The current research aimed to test the use of Mini-FLOTAC for the identification of Eimeria spp. in free-range chickens and compare its results with McMaster. For this study, 40 faecal samples were collected from free-range chickens in a poultry farm located in North-Western Lisbon (Portugal). Each sample was processed with McMaster and Mini-FLOTAC techniques for the detection and count of coccidian Eimeria spp. oocysts. The resulting OPG (oocysts per gram of faeces) data obtained by the two techniques were compared using the Wilcoxon Test and correlated with the Spearman Test, and Mini-FLOTAC’s relative sensitivity was assessed, using a significance level of p<0.05. The average OPG was higher with Mini-FLOTAC and doubled the one obtained using the McMaster method (2669.3 OPG and 1220 OPG, respectively), although these results were not significant. Mini-FLOTAC’s relative sensitivity obtained in this study reached 86% (70.5-95.3%, 95%CI), although this result was not statistically significant. However, correlation of OPG counts between Mini-FLOTAC and McMaster, was significant. These preliminary results suggest the potential interest in the use of Mini-FLOTAC for the diagnosis of coccidiosis by Eimeria spp. in poultry, based on its assessment in a free-range poultry production system.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.issn1582-1366
dc.identifier.urihttp://hdl.handle.net/10400.5/21752
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCIISA (project UIDP/00276/2020, funded by FCT)pt_PT
dc.relationCESAM (project UIDB/AMB/50017/2020, funded by FCT)pt_PT
dc.subjectPoultrypt_PT
dc.subjectfree-rangept_PT
dc.subjectMcMasterpt_PT
dc.subjectMini-FLOTACpt_PT
dc.subjectcoccidiosispt_PT
dc.subjectEimeria spp.pt_PT
dc.titleDiagnosis of coccidiosis by Eimeria spp. in free-range chickens using Mini-FLOTAC and McMaster techniques - preliminary resultspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage18pt_PT
oaire.citation.issue22pt_PT
oaire.citation.startPage13pt_PT
oaire.citation.titleScientia Parasitologicapt_PT
oaire.citation.volume1pt_PT
person.familyNamePestana Lozano
person.familyNameDIAZ ANAYA
person.familyNameOliveira
person.familyNamePaz-Silva
person.familyNameFERREIRA RAMOS NABAIS DE OLIVEIRA REBELO
person.familyNameMadeira de Carvalho
person.givenNameJoão Miguel
person.givenNameADRIANA MARIA
person.givenNameManuela
person.givenNameAdolfo
person.givenNameMARIA TERESA
person.givenNameLuís Manuel
person.identifierA-3772-2013
person.identifier.ciencia-idA81D-BFE3-3D35
person.identifier.ciencia-idBE1E-2B3B-92D2
person.identifier.ciencia-id6D14-E73D-B333
person.identifier.ciencia-id9416-643A-4E0C
person.identifier.orcid0000-0001-5390-6998
person.identifier.orcid0000-0002-8192-6379
person.identifier.orcid0000-0002-3910-1525
person.identifier.orcid0000-0002-0208-3861
person.identifier.orcid0000-0002-2724-2195
person.identifier.orcid0000-0002-7548-667X
person.identifier.ridI-2788-2015
person.identifier.scopus-author-id55928513700
person.identifier.scopus-author-id8253074800
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery477c126a-93dd-43fe-a39f-807a1be17082

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