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Caracterização de correntes de potássio de receptores auditivos de Schistocerca gregaria
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Os processos de sintonia estão descritos, em Insectos, como sendo dependentes das características mecânicas das estruturas auditivas. Contudo, começam a surgir evidências de que mecanismos electrofisiológicos poderão estar envolvidos neste processo. Para esclarecer esta questão, torna-se necessário conhecer as correntes presentes nas células receptoras auditivas. Foi necessário o desenvolvimento de um protocolo para isolar as células do órgão auditivo de Schistocerca gregaria. As correntes de potássio presentes nestas células foram investigadas recorrendo à técnica de Whole-Cell Patch-Clamp. Esta técnica identificar duas correntes de potássio diferentes, uma com parâmetros da activação e inactivação mais rápidos e despolarizados do que a outra. Duas das células estudadas apresentaram apenas uma das duas correntes. Não foi possível identificar o papel destas correntes na sintonia da resposta do órgão auditivo mas o estabelecimento desta técnica poderá permitir novas descrições de correntes de receptores auditivos de Insectos
In Insects, the tuning of the auditory organ is considered to be essentially dependent on the mechanical properties of the peripheral auditory structures. However, evidences pointing to the involvement of electrophysiological mechanisms at the level of the auditory receptor cells are arising. In order to clarify this process, it is necessary to study the currents present in the cells that compose these organs. As a first step it was necessary to develop a protocol to isolate the cells of the hearing organ of Schistocerca gregaria. Then, the potassium currents present in these cells were investigated, using the Whole- Cell Patch-Clamp technique. It was possible to identify two different potassium currents, one with faster and more depolarized activation and inactivation parameters than the other. In two of the studied cells presented only one of the currents was found. Although it was not possible to demonstrate the role of the potassium currents in the tuning of the 4 auditory periphery in this species, the development of the technique may contribute to the description of more currents of auditory receptors of Insects
In Insects, the tuning of the auditory organ is considered to be essentially dependent on the mechanical properties of the peripheral auditory structures. However, evidences pointing to the involvement of electrophysiological mechanisms at the level of the auditory receptor cells are arising. In order to clarify this process, it is necessary to study the currents present in the cells that compose these organs. As a first step it was necessary to develop a protocol to isolate the cells of the hearing organ of Schistocerca gregaria. Then, the potassium currents present in these cells were investigated, using the Whole- Cell Patch-Clamp technique. It was possible to identify two different potassium currents, one with faster and more depolarized activation and inactivation parameters than the other. In two of the studied cells presented only one of the currents was found. Although it was not possible to demonstrate the role of the potassium currents in the tuning of the 4 auditory periphery in this species, the development of the technique may contribute to the description of more currents of auditory receptors of Insects
Descrição
Tese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de Ciências
Palavras-chave
Fisiologia animal Correntes de potássio Audição Teses de mestrado