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Evaluation of the fusion inhibitor P3 peptide as a potential microbicide to prevent HIV transmission in women

2018Journal article Open access
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dc.contributor.authorBártolo, Inês
dc.contributor.authorDiniz, Ana Rita
dc.contributor.authorBorrego, Pedro
dc.contributor.authorFerreira, João Pedro
dc.contributor.authorBronze, MR
dc.contributor.authorBarroso, Helena
dc.contributor.authorPinto, Rui
dc.contributor.authorCardoso, Carlos
dc.contributor.authorPinto, João F.
dc.contributor.authorCeña Diaz, Rafael
dc.contributor.authorBroncano, Pilar Garcia
dc.contributor.authorMuñoz-Fernandez, Maria Angel
dc.contributor.authorTaveira, Nuno
dc.date.accessioned2023-08-23T17:54:05Z
dc.date.available2023-08-23T17:54:05Z
dc.date.issued2018
dc.date.updated2023-02-03T14:18:26Z
dc.description.abstractMicrobicides are an important strategy for preventing the sexual transmission of HIV but, so far, the most advanced tenofovir-based microbicides have had modest efficacy. This has been related to adherence problems and high prevalence of tenofovir-resistant HIV-1 strains. P3 is a new peptide with potent activity against HIV that may be a good microbicide candidate. In this work P3 was formulated in a gel of hydroxyethyl cellulose and its activity, stability and safety profile in Balb/c mice were evaluated. HIV infection was fully blocked by a 1.5% gel containing P3 at the IC90 (366.4 nM) concentration. The antiviral activity did not change at 4°C during 4 months and at 25, 37 and 65°C for 1 week. P3 was stable and fully functional at acidic pH up to 24h, under different concentrations of hydrogen peroxide and in the presence of genital fluids up to 48h. P3 had no antibacterial activity and did not affect sperm motility and vitality. Finally, P3 didn’t cause significant alterations in the vaginal epithelium of Balb/c mice at 0.06 (456.8 μM) and 0.2 mg/day (1522.7 μM) doses. These findings indicate that P3 is an excellent candidate for further development as a microbicide gel for the prevention of HIV transmission in women.pt_PT
dc.description.sponsorshipFinancial support for this research was provided by the Fundação para a Ciência e a Tecnologia (FCT), Portugal (project VIH/SAU/0029/2011), part of the EDCTP2 program supported by the European Union. Inês Bártolo is supported by a post-doc fellowship (SFRH/BPD/76225/2011) from Fundação para a Ciência e Tecnologia (FCT), Portugal. Ana Rita Diniz is supported by a PhD grant (SFRH/BD/89140/2012) from Fundação para a Ciência e Tecnologia (FCT), Portugal. https://www.fct.pt/.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBártolo I, Diniz AR, Borrego P, Ferreira JP, Bronze MR, Barroso H, et al. Evaluation of the fusion inhibitor P3 peptide as a potential microbicide to prevent HIV transmission in women. PLOS ONE [Internet]. 18 de abril de 2018;13(4):e0195744. Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0195744pt_PT
dc.identifier.doi10.1371/journal.pone.0195744pt_PT
dc.identifier.eid2-s2.0-85045888895
dc.identifier.slugcv-prod-1108449
dc.identifier.urihttp://hdl.handle.net/10451/58978
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPlospt_PT
dc.relationDESENVOLVIMENTO DE NOVOS MICROBICIDAS PARA PREVENIR A INFECÇÃO POR VIH
dc.relationDEVELOPMENT AND PRE-CLINICAL EVALUATION OF A NEW MICROBICIDE GEL FOR THE PREVENTION OF HIV-1 AND HIV-2 INFECTION
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0195744pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleEvaluation of the fusion inhibitor P3 peptide as a potential microbicide to prevent HIV transmission in womenpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDESENVOLVIMENTO DE NOVOS MICROBICIDAS PARA PREVENIR A INFECÇÃO POR VIH
oaire.awardTitleDEVELOPMENT AND PRE-CLINICAL EVALUATION OF A NEW MICROBICIDE GEL FOR THE PREVENTION OF HIV-1 AND HIV-2 INFECTION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/VIH%2FSAU%2F0029%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F76225%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F89140%2F2012/PT
oaire.citation.issue4pt_PT
oaire.citation.startPagee0195744pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume13pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameBártolo
person.familyNameBorrego
person.familyNameBeja de Figueiredo Gonzaga Bronze
person.familyNameBarroso
person.familyNameAmaro Pinto
person.familyNamePinto
person.familyNameTaveira
person.givenNameInês
person.givenNamePedro
person.givenNameMaria do Rosário
person.givenNameHelena
person.givenNameRui Manuel
person.givenNameJoão
person.givenNameNuno
person.identifierhttp://www.researcherid.com/rid/I-1376-2012
person.identifier.ciencia-id661E-F5CB-F85A
person.identifier.ciencia-idA71C-0177-722F
person.identifier.ciencia-idEC18-816C-A6BF
person.identifier.ciencia-idE311-7ACE-373D
person.identifier.ciencia-id2B10-96CF-BFC4
person.identifier.ciencia-id4D14-B103-CEC7
person.identifier.ciencia-id7111-3383-A926
person.identifier.orcid0000-0002-2022-8921
person.identifier.orcid0000-0002-1949-9484
person.identifier.orcid0000-0001-5016-4634
person.identifier.orcid0000-0003-4098-5433
person.identifier.orcid0000-0002-1667-7871
person.identifier.orcid0000-0001-6499-310X
person.identifier.orcid0000-0003-0176-5585
person.identifier.ridH-6968-2013
person.identifier.ridC-6595-2008
person.identifier.scopus-author-id25225039400
person.identifier.scopus-author-id35492841400
person.identifier.scopus-author-id7402584819
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid661E-F5CB-F85A | Inês Bártolo
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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