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Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans

2019-04-03Journal article Open access
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dc.contributor.authorMarques, Cátia Filipa Saraiva
dc.contributor.authorBelas, Adriana
dc.contributor.authorAboim, Catarina
dc.contributor.authorCavaco-Silva, Patrícia
dc.contributor.authorTrigueiro, Graça
dc.contributor.authorGama, Luis
dc.contributor.authorPomba, C.
dc.date.accessioned2021-09-29T23:16:22Z
dc.date.available2021-09-29T23:16:22Z
dc.date.issued2019-04-03
dc.descriptionResearch Areas: Microbiologypt_PT
dc.description.abstractThis study aimed to characterize the fecal colonization and sharing of Klebsiella pneumoniae strains between companion animals and humans living in close contact. Fecal samples were collected from 50 healthy participants (24 humans, 18 dogs, and 8 cats) belonging to 18 households. Samples were plated onto MacConkey agar (MCK) plates with and without cefotaxime or meropenem supplementation. Up to five K. pneumoniae colonies per participant were compared by pulsed-field gel electrophoresis (PFGE) after XbaI restriction. K. pneumoniae strains with unique pulse types from each participant were characterized for antimicrobial susceptibility, virulence genes, and multilocus sequence type (MLST). Fecal K. pneumoniae pulse types were compared to those of clinical K. pneumoniae strains from animal and human patients with urinary tract infections (n = 104). K. pneumoniae colonization was detected in nonsupplemented MCK in around 38% of dogs (n = 7) and humans (n = 9). K. pneumoniae strains isolated from dogs belonged to sequence type 17 (ST17), ST188, ST252, ST281, ST423, ST1093, ST1241, ST3398, and ST3399. None of the K. pneumoniae strains were multidrug resistant or hypervirulent. Two households included multiple colonized participants. Notably, two colonized dogs within household 15 (H15) shared a strain each (ST252 and ST1241) with one coliving human. One dog from H16 shared one PFGE-undistinguishable K. pneumoniae ST17 strain with two humans from different households; however, the antimicrobial susceptibility phenotypes of these three strains differed. Two main virulence genotypes were detected, namely fimH-1 mrkD ycfM entB kfu and fimH-1 mrkD ycfM entB kpn. These results highlight the potential role of dogs as a reservoir of K. pneumoniae to humans and vice versa. Furthermore, to our best knowledge, this is the first report of healthy humans and dogs sharing K. pneumoniae strains that were undistinguishable by PFGE/MLST.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMarques C, Belas A, Aboim C, Cavaco-Silva P, Trigueiro G, Gama LT, Pomba C. 2019. Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans [article]. Journal of Clinical Microbiology, 57:6, e01537-18. Doi.org/10.1128/JCM.01537-18.pt_PT
dc.identifier.doi10.1128/JCM.01537-18pt_PT
dc.identifier.eissn1098-660X
dc.identifier.urihttp://hdl.handle.net/10400.5/22110
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relationFEDER funds through the Programa Operacional Factores de Competitividade-COMPETEpt_PT
dc.relation.publisherversionhttps://journals.asm.org/doi/epub/10.1128/JCM.01537-18pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectKlebsiella pneumoniaept_PT
dc.subjectanimal-human sharingpt_PT
dc.subjectclonal relatednesspt_PT
dc.subjectcompanion animalspt_PT
dc.subjectdogpt_PT
dc.subjecthumanspt_PT
dc.titleEvidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humanspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/154629/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/148846/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/48521/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/33465/PT
oaire.citation.conferencePlaceWASHINGTONpt_PT
oaire.citation.issuee01537-18pt_PT
oaire.citation.titleJournal of Clinical Microbiologypt_PT
oaire.citation.volumev. 57 (6)pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
oaire.fundingStreamOE
person.familyNameMarques
person.familyNameInácio Belas
person.familyNameGama
person.familyNameMatias Ferreira Pomba
person.givenNameCátia
person.givenNameAdriana de Jesus
person.givenNameLuis
person.givenNameMaria Constança
person.identifier.ciencia-id8015-B7EE-5FFC
person.identifier.ciencia-id4D1D-1718-6FFF
person.identifier.ciencia-idC71D-140F-E876
person.identifier.ciencia-idDA1D-20A8-7F3C
person.identifier.orcid0000-0001-9648-6380
person.identifier.orcid0000-0001-6564-6839
person.identifier.orcid0000-0003-3894-3488
person.identifier.orcid0000-0002-0504-6820
person.identifier.ridN-4172-2018
person.identifier.scopus-author-id55433369800
person.identifier.scopus-author-id36027387300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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