Publication
High-level expression of IMP-5 carbapenemase owing to point mutation in the -35 promoter region of class 1 integron among Pseudomonas aeruginosa clinical isolates
| dc.contributor.author | Brizio, A | |
| dc.contributor.author | Conceicao, T | |
| dc.contributor.author | Pimentel, M | |
| dc.contributor.author | Da Silva, G | |
| dc.contributor.author | Duarte, A | |
| dc.date.accessioned | 2015-12-30T10:17:57Z | |
| dc.date.available | 2015-12-30T10:17:57Z | |
| dc.date.issued | 2006 | |
| dc.description.abstract | Eight strains of Pseudomonas aeruginosa producing IMP-5 carbapenemases were collected from three Portuguese hospitals. All isolates were epidemiologically unrelated. The bla(IMP-5) gene was inserted into a class I integron previously reported in Acinetobacter baumannii 65FFC. Expression of the the blaIMP-5 gene in P aeruginosa has been shown to be driven by the P, promoter [TTGATA] in which the cytosine was replaced by thymine, which caused an increase in transcription of blaIMP-5 that was confirmed by site-directed mutagenesis. The minimum inhibitory concentrations (MICs) of imipenem for Escherichia coli pGMLA-1 (the recombinant front A. baumannii 65FFC) and E. coli pATG-2 (the recombinant from P. aeruginosa isolates) were 0.5 mg/L and 32 mg/L, respectively. This study reports the spread of a class 1 integron In76 with a new point mutation in the P-1 promoter sequence leading to overproduction of INIP-5. (c) 2005 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved. | |
| dc.format | application/pdf | |
| dc.identifier.citation | INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS. - Vol. 27, n. 1 (JAN 2006), p. 27-31 | |
| dc.identifier.doi | http://dx.doi.org/10.1016/j.ijantimicag.2005.08.023 | |
| dc.identifier.issn | 0924-8579 | |
| dc.identifier.uri | http://hdl.handle.net/10451/21328 | |
| dc.language.iso | eng | |
| dc.publisher | ELSEVIER SCIENCE BV | |
| dc.subject | Infectious Diseases | |
| dc.subject | Microbiology | |
| dc.subject | Pharmacology & Pharmacy | |
| dc.title | High-level expression of IMP-5 carbapenemase owing to point mutation in the -35 promoter region of class 1 integron among Pseudomonas aeruginosa clinical isolates | |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 31 | por |
| oaire.citation.startPage | 27 | por |
| oaire.citation.title | INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | por |
| oaire.citation.volume | Vol. 27 | por |
| rcaap.rights | restrictedAccess | |
| rcaap.type | article |