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Emergence of KPC-3- and OXA-181-producing ST13 and ST17 Klebsiella pneumoniae in Portugal: genomic insights on national and international dissemination

2023-03-31Journal article Restricted access
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dc.contributor.authorElias, Rita
dc.contributor.authorSpadar, Anton
dc.contributor.authorHendrickx, Antoni P A
dc.contributor.authorBonnin, Remy A
dc.contributor.authorDortet, Laurent
dc.contributor.authorPinto, Margarida
dc.contributor.authorPhelan, Jody E
dc.contributor.authorPortugal, Isabel
dc.contributor.authorCampino, Susana
dc.contributor.authorSilva, Gabriela Jorge da
dc.contributor.authorClark, Taane
dc.contributor.authorDuarte, Aida
dc.contributor.authorPerdigão, João
dc.date.accessioned2024-01-19T16:02:36Z
dc.date.available2024-01-19T16:02:36Z
dc.date.issued2023-03-31
dc.date.updated2023-12-29T12:10:30Z
dc.description© The Author(s) 2023. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved.pt_PT
dc.description.abstractBackground Carbapenem-resistant Klebsiella pneumoniae (CRKP) strains are of particular concern, especially strains with mobilizable carbapenemase genes such as blaKPC, blaNDM or blaOXA-48, given that carbapenems are usually the last line drugs in the β-lactam class and, resistance to this sub-class is associated with increased mortality and frequently co-occurs with resistance to other antimicrobial classes. Objectives To characterize the genomic diversity and international dissemination of CRKP strains from tertiary care hospitals in Lisbon, Portugal. Methods Twenty CRKP isolates obtained from different patients were subjected to WGS for species confirmation, typing, drug resistance gene detection and phylogenetic reconstruction. Two additional genomic datasets were included for comparative purposes: 26 isolates (ST13, ST17 and ST231) from our collection and 64 internationally available genomic assemblies (ST13). Results By imposing a 21 SNP cut-off on pairwise comparisons we identified two genomic clusters (GCs): ST13/GC1 (n = 11), all bearing blaKPC-3, and ST17/GC2 (n = 4) harbouring blaOXA-181 and blaCTX-M-15 genes. The inclusion of the additional datasets allowed the expansion of GC1/ST13/KPC-3 to 23 isolates, all exclusively from Portugal, France and the Netherlands. The phylogenetic tree reinforced the importance of the GC1/KPC-3-producing clones along with their rapid emergence and expansion across these countries. The data obtained suggest that the ST13 branch emerged over a decade ago and only more recently did it underpin a stronger pulse of transmission in the studied population. Conclusions This study identifies an emerging OXA-181/ST17-producing strain in Portugal and highlights the ongoing international dissemination of a KPC-3/ST13-producing clone from Portugal.pt_PT
dc.description.sponsorshipThis study was supported in part by UID/DTP/04138/2019 from Fundação para a Ciência e Tecnologia (FCT), Portugal and by Associação para o Desenvolvimento do Ensino e Investigação da Microbiologia (ADEIM). R.E. is supported by the FCT through the PhD Fellowships (Grant Reference: 2021.08701.BD). J.P. (CEECIND/00394/2017) is supported by Fundação para a Ciência e Tecnologia through Estímulo Individual ao Emprego Científico. T.G.C. received funding from the Medical Research Council UK (grant nos. MR/K000551/1, MR/M01360X/1, MR/N010469/1, MR/R020973/1, MR/R025576/1, MR/S01988X/1, MR/S03563X/1, MR/X005895/1) and Biotechnology and Biological Sciences Research Council (BBSRC) UK (BB/R013063/1). S.C. received funding from the Bloomsbury Set, Medical Research Council UK (grants MR/R020973/1, MR/R025576/1, MR/S01988X/1, MR/S03563X/1, MR/X005895/1) and the BBSRC UK (BB/R013063/1).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationElias R, Spadar A, Hendrickx APA, Bonnin RA, Dortet L, Pinto M, et al. Emergence of KPC-3- and OXA-181-producing ST13 and ST17 Klebsiella pneumoniae in Portugal: genomic insights on national and international dissemination. Journal of Antimicrobial Chemotherapy [Internet]. 3 de maio de 2023;78(5):1300–8. Disponível em: https://academic.oup.com/jac/article/78/5/1300/7097591pt_PT
dc.identifier.doi10.1093/jac/dkad093pt_PT
dc.identifier.issn0305-7453
dc.identifier.issn1460-2091
dc.identifier.slugcv-prod-3440104
dc.identifier.urihttp://hdl.handle.net/10451/61986
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Press / British Society for Antimicrobial Chemotherapypt_PT
dc.relationResearch Institute for Medicines
dc.relationExploring the Evolutionary History and Phylogenetic Structure of Klebsiella pneumoniae: translating genomics towards new surveillance frameworks and product development
dc.relationNot Available
dc.relation.publisherversionhttps://academic.oup.com/jac/article-abstract/78/5/1300/7097591pt_PT
dc.titleEmergence of KPC-3- and OXA-181-producing ST13 and ST17 Klebsiella pneumoniae in Portugal: genomic insights on national and international disseminationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleExploring the Evolutionary History and Phylogenetic Structure of Klebsiella pneumoniae: translating genomics towards new surveillance frameworks and product development
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2021.08701.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00394%2F2017%2FCP1476%2FCT0001/PT
oaire.citation.endPage1308pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage1300pt_PT
oaire.citation.titleJournal of Antimicrobial Chemotherapypt_PT
oaire.citation.volume78pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2017
person.familyNameElias
person.familyNameSpadar
person.familyNamePortugal
person.familyNameClark
person.familyNameDuarte
person.familyNameLucas Mota Perdigão
person.givenNameRita
person.givenNameAnton
person.givenNameIsabel
person.givenNameTaane
person.givenNameAida
person.givenNameJoão Ruben
person.identifier642245
person.identifier2524696
person.identifier.ciencia-id4F17-84D2-B49E
person.identifier.ciencia-idE310-5746-C205
person.identifier.ciencia-idDD1B-2564-161C
person.identifier.ciencia-idD517-F4DB-17C1
person.identifier.ciencia-id991C-F26A-9843
person.identifier.orcid0000-0001-6959-9005
person.identifier.orcid0000-0002-1548-1121
person.identifier.orcid0000-0002-7843-0006
person.identifier.orcid0000-0001-8985-9265
person.identifier.orcid0000-0002-0616-4650
person.identifier.orcid0000-0002-0339-1305
person.identifier.ridM-5272-2013
person.identifier.scopus-author-id57220858605
person.identifier.scopus-author-id7403216793
person.identifier.scopus-author-id35495748300
person.identifier.scopus-author-id24723211800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid991C-F26A-9843 | João Ruben Lucas Mota Perdigão
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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