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Assessment of biofilm formation by Campylobacter spp. isolates mimicking poultry slaughterhouse conditions

2022-02Journal article Open access
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dc.contributor.authorAraújo, P. M.
dc.contributor.authorBatista, E.
dc.contributor.authorFernandes, M. H.
dc.contributor.authorFernandes, M. J.
dc.contributor.authorGama, Luis
dc.contributor.authorFraqueza, M. J.
dc.date.accessioned2022-04-22T22:49:17Z
dc.date.available2022-04-22T22:49:17Z
dc.date.issued2022-02
dc.descriptionResearch Areas: Agriculturept_PT
dc.description.abstractThis research aimed to assess the biofilm formation ability of Campylobacter strains under temperature and oxygen stress conditions, similar to those found in the industrial environment, to explain the persistence of this pathogen on the poultry slaughter line. A collection of C. jejuni and C. coli isolates (n = 143) obtained from poultry samples (cecal content and neck skin), collected at slaughterhouse level, from diverse flocks, on different working days, was genotyped by flaA-restriction fragment length polymorphism (RFLP) typing method. A clustering analysis resulted in the assignment of 10 main clusters, from which 15 strains with different flaA-RFLP genotypes were selected for the assessment of biofilm formation ability and antimicrobial susceptibility. Biofilm assays, performed by crystal violet staining method, were conducted with the goal of mimicking some conditions present at the slaughterhouse environment, based on temperature, atmosphere, and contamination levels. Results indicated that many C. jejuni strains with similar flaA-RFLP profiles were present at the slaughterhouse on different processing days. All the strains tested (n = 15) were multidrugresistant except for one. Biofilm formation ability was strain-dependent, and it appeared to have been affected by inoculum concentration, temperature, and tolerance to oxygen levels. At 10 degrees C, adherence levels were significantly lower than at 42 degrees C. Under microaerobic and aerobic atmospheres, at 42 degrees C, 3 strains (C. jejuni 46E, C. jejuni 61C, and C. coli 65B) stood out, exhibiting significant levels of biofilm formation. C. jejuni strains 46E and 61C were inserted in clusters with evidence of persistence at the slaughterhouse for a long period of time. This study demonstrated that Campylobacter strains from broilers are capable of forming biofilms under conditions resembling the slaughterhouse environment. These results should be seen as a cue to improve the programs of hygiene implemented, particularly in those zones that can promote biofilm formation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAraujo PM, Batista E, Fernandes MH, Fernandes MJ, Gama LT, Fraqueza MJ. 2022. Assessment of biofilm formation by Campylobacter spp. isolates mimicking poultry slaughterhouse conditions. Poultry Science, 101(2):101586. Doi 10.1016/j.psj.2021.101586pt_PT
dc.identifier.doi10.1016/j.psj.2021.101586pt_PT
dc.identifier.eissn1525-3171
dc.identifier.urihttp://hdl.handle.net/10400.5/24146
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationGO228 PDR2020-1.0.1-FEADER-PDR2020-101-031254pt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0032579121006076?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCampylobacterpt_PT
dc.subjectPoultrypt_PT
dc.subjectSlaughterhousept_PT
dc.subjectBiofilmpt_PT
dc.subjectFood safetypt_PT
dc.subjectflaA-RFLPpt_PT
dc.titleAssessment of biofilm formation by Campylobacter spp. isolates mimicking poultry slaughterhouse conditionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00276%2F2020/PT
oaire.citation.conferencePlaceAmsterdampt_PT
oaire.citation.titlePoultry Sciencept_PT
oaire.citation.volume101(2):101586 DOI10.1016/j.psj.2021.101586pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGama
person.familyNameFraqueza
person.givenNameLuis
person.givenNameMaria João
person.identifierM-8503-2013
person.identifier.ciencia-idC71D-140F-E876
person.identifier.ciencia-idCA18-CCF2-652B
person.identifier.orcid0000-0003-3894-3488
person.identifier.orcid0000-0002-8388-0511
person.identifier.ridN-4172-2018
person.identifier.scopus-author-id36027387300
person.identifier.scopus-author-id22953692100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione6ff858c-9615-437f-98a7-b61b4362834c
relation.isAuthorOfPublication247d02c1-b9b9-4c96-852b-d03f7bb0a6dd
relation.isAuthorOfPublication.latestForDiscovery247d02c1-b9b9-4c96-852b-d03f7bb0a6dd
relation.isProjectOfPublicationad5a15fb-c850-457e-8ff4-be8af6e13822
relation.isProjectOfPublication.latestForDiscoveryad5a15fb-c850-457e-8ff4-be8af6e13822

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