Publicação
Peptibodies : an elegant solution for a long-standing problem
| dc.contributor.author | Cavaco, Marco | |
| dc.contributor.author | Castanho, Miguel A. R. B. | |
| dc.contributor.author | Neves, Vera | |
| dc.date.accessioned | 2019-03-22T13:47:40Z | |
| dc.date.available | 2019-03-22T13:47:40Z | |
| dc.date.issued | 2018 | |
| dc.description | © 2017 Wiley Periodicals, Inc. | pt_PT |
| dc.description.abstract | Chimeric proteins composed of a biologically active peptide and a fragment crystallizable (Fc) domain of immunoglobulin G (IgG) are known as peptibodies. They present an extended half-life due to neonatal Fc receptor (FcRn) salvage pathway, a decreased renal clearance rate owing to its increased size (≈70 kDa) and, depending on the peptide used in the design of the peptibody, an active-targeting moiety. Also, the peptides therapeutic activity is boosted by the number of peptides in the fusion protein (at least two peptides) and to some peptides’ alterations. Peptibodies are mainly obtained through recombinant DNA technology. However, to improve peptide properties, “unnatural” changes have been introduced to the original peptides’ sequence, for instance, the incorporation of D- or non-natural amino acid residues or even cyclization thus, limiting the application of genetic engineering in the production of peptibodies, since these peptides must be obtained via chemical synthesis. This constrains prompted the development of new methods for conjugation of peptides to Fc domains. Another challenge, subject of intense research, relates to the large-scale production of such peptibodies using these new techniques, which can be minimized by their proved value. To date, two peptibodies, romiplostim and dulaglutide, have been approved and stay as the standard of care in their areas of action. Furthermore, a considerable number of peptibodies are currently in preclinical and clinical development. | pt_PT |
| dc.description.sponsorship | Portuguese Funding Agency, Fundação para a Ciência e a Tecnologia, FCT IP, Grants Numbers: PD/BD/128281/2017, SFRH/ BPD/94466/2013, and PTDC/BBB-NAN/ 1578/2014; Marie Skłodowska-Curie Research and Innovation Staff Exchange (MSCA-RISE), call 20-MSCA-RISE-2014, Grant number: H20 644167–INPACT. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Peptide Science. 2018;110:e23095 | pt_PT |
| dc.identifier.doi | 10.1002/bip.23095 | pt_PT |
| dc.identifier.issn | 2475-8817 | |
| dc.identifier.uri | http://hdl.handle.net/10451/37663 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Wiley-PeptideScience | pt_PT |
| dc.relation | SFRH/ BPD/94466/2013 | pt_PT |
| dc.relation | Trans-BBB peptides for targeting brain metastasis | |
| dc.relation | H20 644167–INPACT. | pt_PT |
| dc.relation.publisherversion | https://onlinelibrary.wiley.com/journal/24758817 | pt_PT |
| dc.subject | Copper-free click chemistry | pt_PT |
| dc.subject | Peptibody | pt_PT |
| dc.subject | Peptide-Fc fusion protein | pt_PT |
| dc.subject | Sortase-mediated protein ligation | pt_PT |
| dc.subject | Streamlined-expressed protein ligation | pt_PT |
| dc.title | Peptibodies : an elegant solution for a long-standing problem | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Trans-BBB peptides for targeting brain metastasis | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//PD%2FBD%2F128281%2F2017/PT | |
| oaire.citation.startPage | e23095. | pt_PT |
| oaire.citation.title | Peptide Science | pt_PT |
| oaire.citation.volume | 110 | pt_PT |
| person.familyName | Cavaco | |
| person.familyName | Castanho | |
| person.familyName | Neves | |
| person.givenName | Marco | |
| person.givenName | Miguel | |
| person.givenName | Vera | |
| person.identifier | 1069324 | |
| person.identifier | 953259 | |
| person.identifier.ciencia-id | 1412-63B8-7494 | |
| person.identifier.ciencia-id | 671C-1860-A160 | |
| person.identifier.orcid | 0000-0002-0938-9038 | |
| person.identifier.orcid | 0000-0001-7891-7562 | |
| person.identifier.orcid | 0000-0002-2989-7208 | |
| person.identifier.rid | O-2176-2018 | |
| person.identifier.scopus-author-id | 56605575600 | |
| person.identifier.scopus-author-id | 26537945300 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | restrictedAccess | pt_PT |
| rcaap.type | article | pt_PT |
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