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Liposomes as a nanoplatform to improve the delivery of antibiotics into Staphylococcus aureus biofilms

2021-03-02Journal article Open access
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dc.contributor.authorFerreira, M.C.
dc.contributor.authorPinto, Sandra N.
dc.contributor.authorAires da Silva, Frederico
dc.contributor.authorBettencourt, Ana
dc.contributor.authorAguiar, Sandra I
dc.contributor.authorGaspar, Maria Manuela
dc.date.accessioned2022-01-31T23:14:17Z
dc.date.available2022-01-31T23:14:17Z
dc.date.issued2021-03-02
dc.descriptionResearch Areas: Pharmacology & Pharmacypt_PT
dc.description.abstractABSTRACT - Staphylococcus aureus biofilm-associated infections are a major public health concern. Current therapies are hampered by reduced penetration of antibiotics through biofilm and low accumulation levels at infected sites, requiring prolonged usage. To overcome these, repurposing antibiotics in combination with nanotechnological platforms is one of the most appealing fast-track and costeffective approaches. In the present work, we assessed the potential therapeutic benefit of three antibiotics, vancomycin, levofloxacin and rifabutin (RFB), through their incorporation in liposomes. Free RFB displayed the utmost antibacterial effect with MIC and MBIC50 below 0.006 µg/mL towards a methicillin susceptible S. aureus (MSSA). RFB was selected for further in vitro studies and the influence of different lipid compositions on bacterial biofilm interactions was evaluated. Although positively charged RFB liposomes displayed the highest interaction with MSSA biofilms, RFB incorporated in negatively charged liposomes displayed lower MBIC50 values in comparison to the antibiotic in the free form. Preliminary safety assessment on all RFB formulations towards osteoblast and fibroblast cell lines demonstrated that a reduction on cell viability was only observed for the positively charged liposomes. Overall, negatively charged RFB liposomes are a promising approach against biofilm S. aureus infections and further in vivo studies should be performed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFerreira M, Pinto SN, Aires-da-Silva F, Bettencourt A, Aguiar SI, Gaspar MM. 2021. Liposomes as a nanoplatform to improve the delivery of antibiotics into Staphylococcus aureus biofilms. Pharmaceutics, 13(3):321. Doi: 10.3390/pharmaceutics13030321pt_PT
dc.identifier.doi10.3390/pharmaceutics13030321pt_PT
dc.identifier.eissn1999-4923
dc.identifier.urihttp://hdl.handle.net/10400.5/23329
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPPBI-POCI-01-0145-FEDER-022122pt_PT
dc.relationNANOFORMULATED HYBRID MOLECULES FOR SPECIFIC TAREGETING MELANOMA METASTASIS
dc.relationPRECISION ONCOLOGY BY INNOVATIVE THERAPIES AND TECHNOLOGIES
dc.relationResearch Institute for Medicines
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationInstitute for Bioengineering and Biosciences
dc.relation.publisherversionhttps://www.mdpi.com/1999-4923/13/3/321pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectBiofilmspt_PT
dc.subjectLiposomespt_PT
dc.subjectInfectionpt_PT
dc.subjectTranswell modelpt_PT
dc.titleLiposomes as a nanoplatform to improve the delivery of antibiotics into Staphylococcus aureus biofilmspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNANOFORMULATED HYBRID MOLECULES FOR SPECIFIC TAREGETING MELANOMA METASTASIS
oaire.awardTitlePRECISION ONCOLOGY BY INNOVATIVE THERAPIES AND TECHNOLOGIES
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMED-QUI%2F31721%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/SAICTPAC%2F0019%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.titlePharmaceuticspt_PT
oaire.citation.volumeVol. 13(3):321pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCatroga Ferreira
person.familyNameAires da Silva
person.familyNameAguiar
person.givenNameMagda Sofia
person.givenNameFrederico
person.givenNameSandra
person.identifier143307
person.identifierA-3500-2009
person.identifier.ciencia-id301A-01AA-1024
person.identifier.ciencia-id4410-6DA0-C74C
person.identifier.ciencia-id8E10-C536-8517
person.identifier.orcid0000-0001-7147-5458
person.identifier.orcid0000-0002-3821-419X
person.identifier.orcid0000-0002-3041-383X
person.identifier.scopus-author-id57195622450
person.identifier.scopus-author-id8519566800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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