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Integrated multi-biomarker responses of juvenile seabass to diclofenac, warming and acidification co-exposure

2018Journal article Restricted access
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dc.contributor.authorLuísa Maulvault, Ana
dc.contributor.authorBarbosa, Vera
dc.contributor.authorAlves, Ricardo
dc.contributor.authorAnacleto, Patrícia
dc.contributor.authorCamacho, Carolina
dc.contributor.authorCunha, Sara
dc.contributor.authorFernandes, José O.
dc.contributor.authorFerreira, Pedro Pousão
dc.contributor.authorRosa, Rui
dc.contributor.authorMarques, António
dc.contributor.authorDiniz, Mário S.
dc.date.accessioned2020-01-19T20:49:42Z
dc.date.available2020-01-19T20:49:42Z
dc.date.issued2018
dc.description.abstractPharmaceutical drugs, such as diclofenac (DCF), are frequently detected in the marine environment, and recent evidence has pointed out their toxicity to non-target marine biota. Concomitantly, altered environmental conditions associated with climate change (e.g. warming and acidification) can also affect the physiology of marine organisms. Yet, the underlying interactions between these environmental stressors (pharmaceutical exposure and climate change-related stressors) still require a deeper understanding. Comprehending the influence of abiotic variables on chemical contaminants' toxicological attributes provides a broader view of the ecological consequences of climate change. Hence, the aim of this study was to assess the ecotoxicological responses of juvenile seabass Dicenthrachus labrax under the co-exposure to DCF (from dietary sources, 500 ± 36 ng kg-1 dw), warming (ΔTºC = +5 °C) and acidification (ΔpCO2 ∼1000 μatm, equivalent to ΔpH = -0.4 units), using an "Integrated Biomarker Response" (IBR) approach. Fish were exposed to these three stressors, acting alone or combined, for 28 days in a full cross-factorial design, and blood, brain, liver and muscle tissues were subsequently collected in order to evaluate: i) animal/organ fitness; ii) hematological parameters and iii) molecular biomarkers. Results not only confirmed the toxicological attributes of dietary exposure to DCF in marine fish species at the tissue (e.g. lower HSI), cellular (e.g. increased ENAs and lower erythrocytes viability) and molecular levels (e.g. increased oxidative stress, protein degradation, AChE activity and VTG synthesis), but also showed that such attributes are altered by warming and acidification. Hence, while acidification and/or warming enhanced some effects of DCF exposure (e.g. by further lowering erythrocyte viability, and increasing brain GST activity and Ub synthesis in muscle), the co-exposure to these abiotic stressors also resulted in a reversion/inhibition of some molecular responses (e.g. lower CAT and SOD inhibition and VTG synthesis). IBRs evidenced that an overall higher degree of stress (i.e. high IBR index) was associated with DCF and warming co-exposure, while the effects of acidification were less evident. The distinct responses observed when DCF acted alone or the animals were co-exposed to the drug together with warming and acidification not only highlighted the relevance of considering the interactions between multiple environmental stressors in ecotoxicological studies, but also suggested that the toxicity of pharmaceuticals can be aggravated by climate change-related stressors (particularly warming), thus, posing additional biological challenges to marine fish populations.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aquatox.2018.06.016pt_PT
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/10451/41212
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0166445X18303606pt_PT
dc.subjectDiclofenacpt_PT
dc.subjectWarmingpt_PT
dc.subjectAcidificationpt_PT
dc.subjectMulti-biomarkerspt_PT
dc.subjectIBRpt_PT
dc.subjectFishpt_PT
dc.titleIntegrated multi-biomarker responses of juvenile seabass to diclofenac, warming and acidification co-exposurept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage79pt_PT
oaire.citation.startPage65pt_PT
oaire.citation.titleAquatic Toxicologypt_PT
oaire.citation.volume202pt_PT
person.familyNameMaulvault
person.familyNameAnacleto
person.familyNameRosa
person.familyNameDiniz
person.givenNameAna Luísa
person.givenNamePatrícia
person.givenNameRui
person.givenNameMario
person.identifier1245458
person.identifier430759
person.identifier108086
person.identifier.ciencia-id9F12-BC2C-5527
person.identifier.ciencia-id9F13-3723-E4B3
person.identifier.ciencia-id2B10-7D61-FF7A
person.identifier.ciencia-idEA1C-766B-258D
person.identifier.orcid0000-0003-4382-1135
person.identifier.orcid0000-0003-3750-9583
person.identifier.orcid0000-0003-2801-5178
person.identifier.orcid0000-0003-1571-0366
person.identifier.ridK-6781-2014
person.identifier.ridH-5933-2013
person.identifier.ridA-4580-2009
person.identifier.ridC-7849-2013
person.identifier.scopus-author-id50461904700
person.identifier.scopus-author-id6507412894
person.identifier.scopus-author-id7102610088
person.identifier.scopus-author-id8980557700
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication6a23e7c0-317a-48f9-b6c2-e0081dd67fa0
relation.isAuthorOfPublicationa98801c6-5d8c-44cf-8b47-5fa0bc9a2f31
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relation.isAuthorOfPublication180f7459-b65c-4db2-b446-1e84540d99ee
relation.isAuthorOfPublication.latestForDiscoverya98801c6-5d8c-44cf-8b47-5fa0bc9a2f31

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