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VIP enhances both pre- and postsynaptic GABAergic transmission to hippocampal interneurones leading to increased excitatory synaptic transmission to CA1 pyramidal cells

2004-11Artigo científico Acesso restrito
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dc.contributor.authorCunha-Reis, Diana
dc.contributor.authorSebastião, Ana M
dc.contributor.authorWirkner, Kerstin
dc.contributor.authorIlles, Peter
dc.contributor.authorRibeiro, Joaquim A.
dc.date.accessioned2021-05-05T14:13:03Z
dc.date.available2021-05-05T14:13:03Z
dc.date.issued2004-11
dc.description© 2004 Nature Publishing Group. All rights reservedpt_PT
dc.description.abstractVasoactive intestinal peptide (VIP) is present in the hippocampus in three subtypes of GABAergic interneurones, two of which innervate preferentially other interneurones, responsible for pyramidal cell inhibition. We investigated how pre- and postsynaptic modulation of GABAergic transmission (to both pyramidal cells and interneurones) by VIP could influence excitatory synaptic transmission in the CA1 area of the hippocampus. VIP (0.1-100 nM) increased [(3)H]GABA release from hippocampal synaptosomes (maximum effect at 1 nM VIP; 63.8 +/- 4.0%) but did not change [(3)H]glutamate release. VIP (0.3-30 nM) enhanced synaptic transmission in hippocampal slices (maximum effect at 1 nM VIP; field excitatory postsynaptic potentials (epsp) slope: 23.7 +/- 1.1%; population spike amplitude: 20.3 +/- 1.7%). The action on field epsp slope was fully dependent on GABAergic transmission since it was absent in the presence of picrotoxin (50 microM) plus CGP55845 (1 microM). VIP (1 nM) did not change paired-pulse facilitation but increased paired-pulse inhibition in CA1 pyramidal cells (16.0 +/- 0.9%), reinforcing the involvement of GABAergic transmission in the action of VIP. VIP (1 nM) increased muscimol-evoked inhibitory currents by 36.4 +/- 8.7% in eight out of ten CA1 interneurones in the stratum radiatum. This suggests that VIP promotes increased inhibition of interneurones that control pyramidal cells, leading to disinhibition of synaptic transmission to pyramidal cell dendrites. In conclusion, concerted pre- and postsynaptic actions of VIP lead to disinhibition of pyramidal cell dendrites causing an enhancement of synaptic transmission.pt_PT
dc.description.sponsorshipDiana Cunha‐Reis was in receipt of an FCT PhD fellowship. This work was supported by FCT and Deutsche Forschungsgemeinschaft (IL‐20/9‐2).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBr J Pharmacol. 2004 Nov;143(6):733-744pt_PT
dc.identifier.doi10.1038/sj.bjp.0705989pt_PT
dc.identifier.eissn1476-5381
dc.identifier.issn0007-1188
dc.identifier.urihttp://hdl.handle.net/10451/47659
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley & Sons, Inc.pt_PT
dc.relation.publisherversionhttps://bpspubs.onlinelibrary.wiley.com/journal/14765381pt_PT
dc.subjectVIPpt_PT
dc.subjectGABApt_PT
dc.subjectInterneuronespt_PT
dc.subjectHippocampuspt_PT
dc.subjectRatpt_PT
dc.subjectSlicespt_PT
dc.subjectPatch clamppt_PT
dc.subjectSynaptosomespt_PT
dc.titleVIP enhances both pre- and postsynaptic GABAergic transmission to hippocampal interneurones leading to increased excitatory synaptic transmission to CA1 pyramidal cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage744pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage733pt_PT
oaire.citation.titleBritish Journal of Pharmacologypt_PT
oaire.citation.volume143pt_PT
person.familyNameJerónimo da Cunha Reis
person.familyNameSebastião
person.familyNameRibeiro
person.givenNameDiana Lina
person.givenNameAna M
person.givenNameJoaquim
person.identifierF-1689-2011
person.identifier.ciencia-id1F1E-73C0-FD44
person.identifier.ciencia-idF112-55E8-E37E
person.identifier.ciencia-id081F-2518-907F
person.identifier.orcid0000-0002-0900-9306
person.identifier.orcid0000-0001-9030-6115
person.identifier.orcid0000-0002-9330-3507
person.identifier.scopus-author-id8571270200
person.identifier.scopus-author-id7004409879
person.identifier.scopus-author-id35498669400
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbd9c582c-30c6-4cf5-b91a-143562ce0931
relation.isAuthorOfPublication304abd7f-071b-4447-a8a3-4aa5f0547141
relation.isAuthorOfPublication86da944c-5e6a-4ec5-a56e-4ed82ece7a17
relation.isAuthorOfPublication.latestForDiscovery304abd7f-071b-4447-a8a3-4aa5f0547141

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