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Publicação
Development of an analytical method for the simultaneous detection of bisphenols using a Surface-enhanced Raman scattering quantitative approach
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Resumo(s)
In recent years, Raman spectroscopy presented itself as one of the analytical methodologies with most potential, with SERS leading the way. The use of metal nanoparticles promotes an immense increase in the intensity obtained and allows a substantial reduction in the limit of quantification, raising SERS to the same level as reference methods such as HPLC. In this work, we want to demonstrate the powerful applications of SERS by attempting the development of an analytical method for the detection and quantification of important molecules in daily-life samples. Bisphenols are small estrogen-like molecules present in a broad variety of consumer goods such as plastics, tin cans, thermal papers and many others. And with their toxicity profile not yet fully established but pointing to genotoxic danger, it is very important to develop a simple, fast and very sensitive method for their detection. In this work it was developed a SERS method capable of detect and quantify three different bisphenols, either alone and in mixtures, while drawing calibration curves with correlations up to 0,99. It was also possible to perform analysis in different matrices with simple changes in the sample preparation procedure, increasing the versatility of the method. Those results progressed into the analysis of real samples and it was possible to detect the presence of bisphenols in thermal paper and store's tickets. It was, then, possible to develop a green, very fast and very sensitive method for detection of bisphenols, capable of matching the renowned methods of reference, hoping it will help shedding some spotlight on SERS's wonderful advantages.
Descrição
Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2014
Palavras-chave
Mestrado Integrado - 2014 Raman spectroscopy SERS Bisphenol