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PCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhouses

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dc.contributor.authorSeabra, Sofia G.
dc.contributor.authorMartins, Joana
dc.contributor.authorBrás, Patrícia
dc.contributor.authorTavares, Ana M.
dc.contributor.authorFreitas, Inês
dc.contributor.authorBarata, António
dc.contributor.authorRebelo, Maria Teresa
dc.contributor.authorMateus, Célia
dc.contributor.authorPaulo, Octávio S.
dc.contributor.authorFigueiredo, Elisabete
dc.date.accessioned2021-12-10T11:29:57Z
dc.date.available2021-12-10T11:29:57Z
dc.date.issued2021
dc.descriptionOriginal articlept_PT
dc.description.abstractThe tiger-fl y Coenosia attenuata Stein (Diptera: Muscidae: Coenosiini) is a generalist predator that preys on several pests of greenhouse crops and is considered a biological control agent in the Mediterranean region. Previous behavioural observations identifi ed its preferred prey, but a more in-depth evaluation will benefi t from using Polymerase Chain Reaction amplifi cation of prey DNA remains in the gut of this predator. To evaluate the rate of decay and suitability of this method for use in the fi eld assessments, we carried out a laboratory feeding calibration experiment on 355 females of C. attenuata, which were killed at different intervals of time after ingestion (10 time points from 0 to 48 h). The prey species tested were: Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae: Trialeurodini), Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae), Diglyphus isaea (Walker) (Hymenoptera: Eulophidae: Cirrospilini), Bradysia impatiens (Johannsen) (Diptera: Sciaridae) and Drosophila mercatorum Patterson & Wheeler (Diptera: Drosophilidae: Drosophilini). Based on a probit model, amplifi cation success of prey DNA declined exponentially with increasing time after ingestion. The half-time molecular detection differed between species, ranging from an average of 5 h for T. vaporariorum and D. isaea, 6 h for B. impatiens, 15 h for L. huidobrensis to more than 40 h for D. mercatorum. This study confi rmed the feasibility of using DNA based detection to identify prey species in the gut contents of C. attenuata and provided calibration curves for a better understanding of predation activity in this agroecosystempt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEur. J. Entomol. 118: 335–343, 2021pt_PT
dc.identifier.doi10.14411/eje.2021.035pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/22689
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstitute of Entomology, Biology Centre, CASpt_PT
dc.relationCentre for Ecology, Evolution and Environmental Changes
dc.relationLinking Landscape, Environment, Agriculture and Food
dc.relation.publisherversionhttp://www.eje.czpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDipterapt_PT
dc.subjectMuscidaept_PT
dc.subjectCoenosia attenuatapt_PT
dc.subjectpredationpt_PT
dc.subjectgut contentspt_PT
dc.subjectprey molecular identifi cationpt_PT
dc.subjectPolymerase Chain Reactionpt_PT
dc.subjectprobit modelspt_PT
dc.subjecthalf-time molecular detectionpt_PT
dc.titlePCR-based detection of prey DNA in the gut contents of thr tiger-fly, Coenosia attenuata (Diptera: Muscidae), a biological control agent in Mediterranean greenhousespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Ecology, Evolution and Environmental Changes
oaire.awardTitleLinking Landscape, Environment, Agriculture and Food
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F099723%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIA%2F00329%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04129%2F2020/PT
oaire.citation.titleEuropean Journal of Entomologypt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFigueiredo
person.givenNameElisabete
person.identifier.ciencia-idD217-1213-E402
person.identifier.orcid0000-0002-0795-4216
person.identifier.scopus-author-id15044208800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication455e95e9-a413-47b5-a462-e1f78a6ca73d
relation.isAuthorOfPublication.latestForDiscovery455e95e9-a413-47b5-a462-e1f78a6ca73d
relation.isProjectOfPublicatione509a29d-0ed2-44be-9cb4-6a215934567d
relation.isProjectOfPublicationeddea404-0134-44ab-852e-f1e0ec6516ff
relation.isProjectOfPublication242ffd71-e775-4d6a-8b04-1559d7f37ad1
relation.isProjectOfPublication.latestForDiscovery242ffd71-e775-4d6a-8b04-1559d7f37ad1

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