Repository logo
 
Publication

Evaluation of a biocide used in the Biological Isolation and Containment Unit of a Veterinary Teaching Hospital

2021-05-27Journal article Open access
Simple item page
dc.contributor.authorGERALDES, CATARINA
dc.contributor.authorVerdial, Cláudia
dc.contributor.authorCunha, Eva
dc.contributor.authorAlmeida, Virgilio
dc.contributor.authorTavares, Luis
dc.contributor.authorOliveira, Manuela
dc.contributor.authorGil, Solange
dc.date.accessioned2021-11-09T12:09:07Z
dc.date.available2021-11-09T12:09:07Z
dc.date.issued2021-05-27
dc.descriptionResearch Areas: Infectious Diseases ; Pharmacology & Pharmacypt_PT
dc.description.abstractHospital-acquired infections (HAIs) are a rising problem worldwide, and the best way of coping with them is through infection tracking and surveillance systems, combined with prevention strategies, namely efficient disinfection protocols, that employ various biocides. However, increasing reports about reductions in biocide susceptibility and the development of cross-resistance to antimicrobials emphasize the need for identifying the factors influencing biocide efficiency. In this study, 29 bacterial isolates (n = 3 E. coli, n = 2 Pseudomonas spp., n = 23 Enterococcus spp., and n = 1 Staphylococcus pseudintermedius), obtained from environmental samples collected from the Biological Isolation and Containment Unit (BICU), of the Veterinary Teaching Hospital of the Faculty of Veterinary Medicine, University of Lisbon, were tested in order to determine their antimicrobial susceptibility to various antibiotics. Thirteen of these isolates were further selected in order to determine their antimicrobial susceptibility to Virkon™ S, with and without the presence of organic matter. Afterward, seven of these isolates were incubated in the presence of sub-lethal concentrations of this formulation and, subsequently, new susceptibility profiles were determined. Fourteen of the 29 isolates (48.3%) were classified as multidrug resistant, all previously identified as enterococci. Concerning Virkon™ S’s susceptibility, the Minimal Bactericidal Concentration (MBC) of this biocide regarding all isolates was at least eight times lower than the concentration regularly used, when no organic matter was present. However, when organic matter was added, MBC values rose up to 23 times. After exposure to sub-lethal concentrations of Virkon™ S, four enterococci presented a phenotypical change regarding antimicrobial susceptibility towards gentamicin. Virkon™ S also resulted in higher MBC values, up to 1.5 times, in the presence of low concentrations of organic matter, but no rise in these values was observed in assays without interfering substance. Virkon™ S seemed to be an efficient formulation in eliminating all bacteria isolates isolated from the BICU. However, organic matter could represent a hindrance to this ability, which emphasizes the importance of sanitization before disinfection procedures. The changes seen in antimicrobial susceptibility could be explained by a general stress-induced response promoted by the sub-lethal levels of Virkon™ S. Additionally, when no organic matter was present, a decrease in susceptibility to this biocide seemed to be non-existent.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGeraldes C, Verdial C, Cunha E, Almeida V, Tavares L, Oliveira M, Gil S. 2021. Evaluation of a biocide used in the Biological Isolation and Containment Unit of a Veterinary Teaching Hospital. Antibiotics, 10(6):639. DOI:10.3390/antibiotics10060639pt_PT
dc.identifier.doi10.3390/antibiotics10060639pt_PT
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10400.5/22533
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relation.publisherversionhttps://www.mdpi.com/2079-6382/10/6/639pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiocidept_PT
dc.subjectVirkon™ Spt_PT
dc.subjectSusceptibilitypt_PT
dc.subjectBiological Isolation and Containment Unitpt_PT
dc.subjectantibioticpt_PT
dc.titleEvaluation of a biocide used in the Biological Isolation and Containment Unit of a Veterinary Teaching Hospitalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.issue639pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume10(6)pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDA SILVA GERALDES
person.familyNameGonçalves da Cunha
person.familyNameAlmeida
person.familyNameMorgado Tavares
person.familyNameOliveira
person.familyNameGil Neves
person.givenNameCATARINA
person.givenNameEva Sofia
person.givenNameVirgilio
person.givenNameLuis Manuel
person.givenNameManuela
person.givenNameSolange
person.identifier.ciencia-idF314-2662-E021
person.identifier.ciencia-id9811-7B6B-E2B9
person.identifier.ciencia-id4212-3A73-3484
person.identifier.ciencia-id9011-18C6-482D
person.identifier.ciencia-idBE1E-2B3B-92D2
person.identifier.ciencia-id2E16-4021-64AB
person.identifier.orcid0000-0002-7885-1820
person.identifier.orcid0000-0002-5641-4400
person.identifier.orcid0000-0001-8671-6285
person.identifier.orcid0000-0002-3910-1525
person.identifier.orcid0000-0001-9565-6261
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication92cfc988-8d0d-4c43-930c-6d9b0ca149bf
relation.isAuthorOfPublication6177b175-6c2b-4b46-b383-f521ae7880cc
relation.isAuthorOfPublicationca97db03-0539-45d2-9406-d9fa4e15f8e7
relation.isAuthorOfPublication1afd8082-0715-4fa2-8314-d2d7de3fa937
relation.isAuthorOfPublicationabd012d1-0b2c-43f6-ba34-5f65c785a479
relation.isAuthorOfPublication6daabcd3-7d3f-47f8-9ef7-aee7458d21e0
relation.isAuthorOfPublication.latestForDiscovery6177b175-6c2b-4b46-b383-f521ae7880cc
relation.isProjectOfPublication8563a044-0c86-49a8-a275-8b1bb62cc0d8
relation.isProjectOfPublication.latestForDiscovery8563a044-0c86-49a8-a275-8b1bb62cc0d8

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Evaluation of a Biocide Used in the Biological Isolation and Containment Unit of a Veterinary Teaching Hospital.pdf
Size:
703.63 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIISA - Artigos em revistas internacionais