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How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico and in vivo models

2020-04-27Artigo científico Acesso aberto
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dc.contributor.authorAmaral, Mariana
dc.contributor.authorMartins, Ana Sofia
dc.contributor.authorCatarino, José
dc.contributor.authorFaísca, Pedro
dc.contributor.authorKumar, Pradeep
dc.contributor.authorPinto, João F.
dc.contributor.authorPinto, Rui
dc.contributor.authorCorreia, Isabel
dc.contributor.authorAscensão, Lia
dc.contributor.authorAfonso, Ricardo A.
dc.contributor.authorGaspar, Catarina
dc.contributor.authorCharmier, Adília J.
dc.contributor.authorFigueiredo, Isabel Vitória
dc.contributor.authorReis, Catarina Pinto
dc.date.accessioned2022-03-07T16:26:16Z
dc.date.available2022-03-07T16:26:16Z
dc.date.issued2020-04-27
dc.date.updated2022-02-21T09:24:42Z
dc.description.abstractCurrently, insulin can only be administered through the subcutaneous route. Due to the flaws associated with this route, it is of interest to orally deliver this drug. However, insulin delivered orally has several barriers to overcome as it is degraded by the stomach’s low pH, enzymatic content, and poor absorption in the gastrointestinal tract. Polymers with marine source like chitosan are commonly used in nanotechnology and drug delivery due to their biocompatibility and special features. This work focuses on the preparation and characterization of mucoadhesive insulin-loaded polymeric nanoparticles. Results showed a suitable mean size for oral administration (<600 nm by dynamic laser scattering), spherical shape, encapsulation efficiency (59.8%), and high recovery yield (80.6%). Circular dichroism spectroscopy demonstrated that protein retained its secondary structure after encapsulation. Moreover, the mucoadhesive potential of the nanoparticles was assessed in silico and the results, corroborated with ex-vivo experiments, showed that using chitosan strongly increases mucoadhesion. Besides, in vitro and in vivo safety assessment of the final formulation were performed, showing no toxicity. Lastly, the insulin-loaded nanoparticles were effective in reducing diabetic rats’ glycemia. Overall, the coating of insulin-loaded nanoparticles with chitosan represents a potentially safe and promising approach to protect insulin and enhance peroral delivery.pt_PT
dc.description.sponsorshipSupported in part by UID/DTP/04138/2019 from FCT, Portugal and DREAMS (ULHT). SEM analysis was funded by FCT/MCTES for the financial support to CESAM (UIDP/50017/2020+UIDB/50017/2020), through national funds. Acknowledgments: The authors are grateful to the Carla Vânia (iMedUlisboa) for her collaboration in HPLC analysis and Joana Moreira (ECTS-ULHT) for her collaboration in conducting some experiments.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmaral M, Martins AS, Catarino J, Faísca P, Kumar P, Pinto JF, et al. How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico, and in vivo models. Biomolecules [Internet]. 2020;10(5):675. Disponível em: https://www.mdpi.com/2218-273X/10/5/675pt_PT
dc.identifier.doidoi.org/10.3390/biom10050675pt_PT
dc.identifier.eid2-s2.0-85083970308
dc.identifier.issn2218-273X
dc.identifier.slugcv-prod-1634277
dc.identifier.urihttp://hdl.handle.net/10451/51634
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationResearch Institute for Medicines
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relation.publisherversionhttps://www.mdpi.com/2218-273X/10/5/675pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMarine-derived biomoleculespt_PT
dc.subjectDiabetes mellituspt_PT
dc.subjectInsulinpt_PT
dc.subjectMucoadhesionpt_PT
dc.subjectNanoparticlept_PT
dc.subjectOral deliverypt_PT
dc.titleHow can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico and in vivo modelspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.citation.issue5pt_PT
oaire.citation.startPage675pt_PT
oaire.citation.titleBiomoleculespt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMoura das Neves Amaral
person.familyNamePinto
person.familyNameAfonso
person.familyNameGaspar
person.familyNameBeco Pinto Reis
person.givenNameMariana
person.givenNameJoão
person.givenNameRicardo
person.givenNameCatarina
person.givenNameAna Catarina
person.identifierAAE-5678-2021
person.identifierM-8132-2013
person.identifier.ciencia-idCE17-A249-4380
person.identifier.ciencia-id4D14-B103-CEC7
person.identifier.ciencia-id6312-CA62-B6C3
person.identifier.ciencia-id9F19-CA9F-1462
person.identifier.ciencia-id1317-A7D6-3B1C
person.identifier.orcid0000-0002-4526-2157
person.identifier.orcid0000-0001-6499-310X
person.identifier.orcid0000-0001-5990-4539
person.identifier.orcid0000-0002-3617-7057
person.identifier.orcid0000-0002-1046-4031
person.identifier.ridC-6595-2008
person.identifier.ridD-5515-2019
person.identifier.ridH-7256-2015
person.identifier.scopus-author-id57216590315
person.identifier.scopus-author-id7402584819
person.identifier.scopus-author-id6603692673
person.identifier.scopus-author-id57198788395
person.identifier.scopus-author-id14027161300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaidCE17-A249-4380 | Mariana Moura das Neves Amaral
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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