Repository logo
 
Publication

Differences in mechanisms of stress tolerance induction between strains of monogonont rotifer Brachionus sp.

2024-06Doctoral thesis Embargoed access
Simple item page
datacite.subject.fosCiências Naturais::Ciências Biológicaspt_PT
dc.contributor.advisorFonseca, Vanessa
dc.contributor.advisorBossier, Peter
dc.contributor.advisorNovais, Sara
dc.contributor.authorGranada, Luana
dc.date.accessioned2025-02-24T17:56:19Z
dc.date.embargo2026-07
dc.date.issued2024-06
dc.date.submitted2023-09
dc.description.abstractAquaculture production is an important food source for human consumption worldwide, benefiting from methodologies that enable the development of advantageous phenotypic traits and, consequently, the reduction of biological and economic losses. Organisms exposed to environmental stimuli at early life stages can undergo molecular responses associated to increased tolerance to abiotic and biotic stressors. Rotifer Brachionus sp. has been crucial for the development of sustainable aquaculture practices, since it is used as first live food for several species’ larvae, allowing a stable production of aquatic seedling. Moreover, it has been used as model organism in several research fields, due to characteristics such as short life cycle and ease of handling. For these reasons, two strains of B. koreanus were used in this thesis to understand the potential of heat stress in early stages of development to induce tolerance to several environmental stressors later in life, and to study possible molecular mechanisms involved in this phenotypic alteration. Although genetically and morphologically identical, MRS10 and IBA3 strains showed differences in several life parameters and in tolerance to various stressors. Overall, the non-lethal heat shock (NLHS) conditions determined in this thesis proved to successfully induce tolerance to heat stress, high salinity, hydrogen peroxide, and cadmium chloride in rotifers. Induced thermotolerance was concomitant with up-regulation of several hsp genes and increased production of HSP70, whilst epigenetic alterations showed to have a major role in the development of cross-tolerance mediated by NLHS, with up-regulation of genes related to histone H3 modifications, and increased levels of total acetylation of histone H3. This thesis demonstrated the potential of Brachionus sp. as model organism to study molecular mechanisms underlying induced tolerance, and of NLHS as promising method to improve culture conditions of rotifers, with clear advantages for the aquaculture sector.pt_PT
dc.identifier.tid101540701pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/98690
dc.language.isoengpt_PT
dc.relationEpiStress - Epigenetic mechanisms of stress tolerance in Nereis diversicolor
dc.subjectAquaculturept_PT
dc.subjectCross-tolerancept_PT
dc.subjectEpigenetic mechanismspt_PT
dc.subjectHeat shock proteinspt_PT
dc.subjectNon-lethal heat shockpt_PT
dc.titleDifferences in mechanisms of stress tolerance induction between strains of monogonont rotifer Brachionus sp.pt_PT
dc.typedoctoral thesis
dspace.entity.typePublication
oaire.awardTitleEpiStress - Epigenetic mechanisms of stress tolerance in Nereis diversicolor
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F102036%2F2014/PT
person.familyNameGranada Almeida
person.givenNameLuana
person.identifierU-5644-2018
person.identifier.ciencia-id431A-F712-709B
person.identifier.orcid0000-0002-1382-9243
person.identifier.scopus-author-id56513933800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typedoctoralThesispt_PT
relation.isAuthorOfPublicationaafe6caf-6826-44ef-8e4b-3db9c5a548e0
relation.isAuthorOfPublication.latestForDiscoveryaafe6caf-6826-44ef-8e4b-3db9c5a548e0
relation.isProjectOfPublicationf5502d53-7a2e-4a79-92ae-c910fbf7964e
relation.isProjectOfPublication.latestForDiscoveryf5502d53-7a2e-4a79-92ae-c910fbf7964e
thesis.degree.nameTese de doutoramento, Biologia (Biologia Marinha e Aquacultura ), Universidade de Lisboa, Faculdade de Ciências, 2024pt_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
scnd990026354742078_td_Luana_Granada.pdf
Size:
2.37 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.2 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FC - Teses de Doutoramento