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Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals

2021-06-17Journal article Open access
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dc.contributor.authorLawal, Opeyemi U.
dc.contributor.authorFraqueza, M. J.
dc.contributor.authorWorning, Peder
dc.contributor.authorBouchami, Ons
dc.date.accessioned2022-02-06T22:43:12Z
dc.date.available2022-02-06T22:43:12Z
dc.date.issued2021-06-17
dc.descriptionResearch Areas: Microbiology ; Pharmacology & Pharmacypt_PT
dc.description.abstractStaphylococcus saprophyticus is a common pathogen of the urinary tract, a heavy metal-rich environment, but information regarding its heavy metal resistance is unknown. We investigated 422 S. saprophyticus isolates from human infection and colonization/contamination, animals, and environmental sources for resistance to copper, zinc, arsenic, and cadmium using the agar dilution method. To identify the genes associated with metal resistance and assess possible links to pathogenicity, we accessed the wholegenome sequence of all isolates and used in silico and pangenome-wide association approaches. The MIC values for copper and zinc were uniformly high (1,600mg/liter). Genes encoding copper efflux pumps (copA, copB, copZ, mco, and csoR) and zinc transporters (zinT, czrAB, znuBC, and zur) were abundant in the population (20 to 100%). Arsenic and cadmium showed various susceptibility levels. Genes encoding the ars operon (arsRDABC), an ABC transporter and a two-component permease, were linked to resistance to arsenic (MICs$ 1,600mg/liter; 14% [58/422]; P, 0.05). At least three cad genes (cadA or cadC and cadD-cadX or czrC) and genes encoding multidrug efflux pumps and hyperosmoregulation in acidified conditions were associated with resistance to cadmium (MICs$ 200mg/liter; 20% [85/422]; P, 0.05). These resistance genes were frequently carried by mobile genetic elements. Resistance to arsenic and cadmium were linked to human infection and a clonal lineage originating in animals (P, 0.05). Altogether, S. saprophyticus was highly resistant to heavy metals and accumulated multiple metal resistance determinants. The highest arsenic and cadmium resistance levels were associated with infection, suggesting resistance to these metals is relevant for S. saprophyticus pathogenicitypt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLawal OU, Fraqueza MJ, Worning P, Bouchami O, Bartels MD, Goncalves L, Paixão P, Goncalves E, Toscano C, Empel J, Urbans M, Domínguez MA, Westh H, de Lencastre H, Miragaia M. 2021. Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals. Antimicrobial Agents Chemotherapy, 65(7):e02685-20. Doi: 10.1128/AAC.02685-20pt_PT
dc.identifier.doi10.1128/AAC.02685-20pt_PT
dc.identifier.eissn1098-6596
dc.identifier.issn0066-4804
dc.identifier.urihttp://hdl.handle.net/10400.5/23413
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relationLISBOA-01-0145-FEDER-007660pt_PT
dc.relationApplied Molecular Biosciences Unit
dc.relation.publisherversionhttps://journals.asm.org/doi/epub/10.1128/AAC.02685-20pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectStaphylococcus saprophyticuspt_PT
dc.subjectUrinary tract infectionpt_PT
dc.subjectEnvironmentpt_PT
dc.subjectHeavy metalspt_PT
dc.subjectWhole-genome sequencingpt_PT
dc.subjectPan-GWASpt_PT
dc.subjectMetal resistance determinantspt_PT
dc.subjectCopperpt_PT
dc.subjectZincpt_PT
dc.subjectArsenicpt_PT
dc.subjectCadmiumpt_PT
dc.subjectMetal resistancept_PT
dc.titleStaphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metalspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04378%2F2019/PT
oaire.citation.conferencePlaceWashingtonpt_PT
oaire.citation.titleAntimicrobial Agent and Chemotherapy: Epidemiology and Surveillancept_PT
oaire.citation.volumeVol.65(7):e02685-20pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFraqueza
person.givenNameMaria João
person.identifierM-8503-2013
person.identifier.ciencia-idCA18-CCF2-652B
person.identifier.orcid0000-0002-8388-0511
person.identifier.scopus-author-id22953692100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication247d02c1-b9b9-4c96-852b-d03f7bb0a6dd
relation.isAuthorOfPublication.latestForDiscovery247d02c1-b9b9-4c96-852b-d03f7bb0a6dd
relation.isProjectOfPublication8325b928-9908-40e3-b06a-c4cd3d6adede
relation.isProjectOfPublication.latestForDiscovery8325b928-9908-40e3-b06a-c4cd3d6adede

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