Logo do repositório
 
Publicação

vCPP2319 interacts with metastatic breast cancer extracellular vesicles (EVs) and transposes a human blood-brain barrier model

2024Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorOliveira, Filipa
dc.contributor.authorCavaco, Marco
dc.contributor.authorFigueira, Tiago Nascimento
dc.contributor.authorNapoleão, Patricia
dc.contributor.authorValle, Javier
dc.contributor.authorNeves, Vera
dc.contributor.authorAndreu, David
dc.contributor.authorCastanho, Miguel A. R. B.
dc.date.accessioned2025-06-11T14:38:01Z
dc.date.available2025-06-11T14:38:01Z
dc.date.issued2024
dc.description© 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/)pt_PT
dc.description.abstractBrain metastases (BM) are frequently found in cancer patients and, though their precise incidence is difficult to estimate, there is evidence for a correlation between BM and specific primary cancers, such as lung, breast, and skin (melanoma). Among all these, breast cancer is the most frequently diagnosed among women and, in this case, BM cause a critical reduction of the overall survival (OS), especially in triple negative breast cancer (TNBC) patients. The main challenge of BM treatment is the impermeable nature of the blood-brain barrier (BBB), which shields the central nervous systems (CNS) from chemotherapeutic drugs. Extracellular vesicles (EVs) have been proposed as ideal natural drug carriers and these may exhibit some advantages over synthetic nanoparticles (NPs). In this work, we isolate breast cancer-derived EVs and study their ability to carry vCPP2319, a peptide with dual cell-penetration and anticancer activities. The selective cytotoxicity of anticancer peptide-loaded EVs towards breast cancer cells and their ability to translocate an in vitro BBB model are also addressed. Overall, it was possible to conclude that vCPP2319 naturally interacts with breast cancer-derived EVs, being retained at the surface of these vesicles. Moreover, the results revealed a cytotoxic activity for peptide-loaded EVs similar to that obtained with the peptide alone and the ability of peptide-loaded EVs to translocate an in vitro BBB model, which contrasts with the results obtained with the peptide alone. In conclusion, this work supports the use of EVs in the development of biological drug-delivery systems (DDS) capable of translocating the BBB.pt_PT
dc.description.sponsorshipFDO and MC acknowledge FCT I.P. for fellowships PD/BD/135046/2017, PD/BD/128281/2017. FCT I.P. is also acknowledged for funding (Projects PTDC/BIA-BQM/5027/2020 and PTDC/BTM-MAT/2472/2021). Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE) is also acknowledged for funding: call H2020-MSCA-RISE-2014, grant agreement 644167, 2015–2019. The project leading to these results have received funding from “la Caixa” Foundation (ID 100010434) and FCT, I.P under the agreement LCF/PR/HP21/52310015. Work at UPF was supported by grant AGL2017-84097-C2-2-R and the “María de Maeztu” Program for Units of Excellence in R&D (MDM-2014-0370) from the Spanish Ministry of Economy and Competitiveness (MINECO).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHeliyon. 2024 Dec 4;10(23):e40907pt_PT
dc.identifier.doi10.1016/j.heliyon.2024.e40907pt_PT
dc.identifier.eissn2405-8440
dc.identifier.urihttp://hdl.handle.net/10400.5/101497
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationLCF/PR/HP21/52310015pt_PT
dc.relationExosomes as drug delivery systems in metastatic breast cancer
dc.relationTrans-BBB peptides for targeting brain metastasis
dc.relationTRESPASS2TREAT: A novel approach in pharmacotherapies for breast cancer patients with brain metastasis.
dc.relationInnovative peptides against cancer and pathogenic bacteria, with advances in science, biopharmaceutical drug development, product market targeting, training , and communication.
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/heliyonpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectAnticancer peptidept_PT
dc.subjectBlood-brain barrierpt_PT
dc.subjectBrain metastasespt_PT
dc.subjectDrug-delivery systemspt_PT
dc.subjectExtracellular vesiclespt_PT
dc.subjectMetastatic breast cancerpt_PT
dc.titlevCPP2319 interacts with metastatic breast cancer extracellular vesicles (EVs) and transposes a human blood-brain barrier modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleExosomes as drug delivery systems in metastatic breast cancer
oaire.awardTitleTrans-BBB peptides for targeting brain metastasis
oaire.awardTitleTRESPASS2TREAT: A novel approach in pharmacotherapies for breast cancer patients with brain metastasis.
oaire.awardTitleInnovative peptides against cancer and pathogenic bacteria, with advances in science, biopharmaceutical drug development, product market targeting, training , and communication.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F135046%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F128281%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2020/PTDC%2FBIA-BQM%2F5027%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-MAT%2F2472%2F2021/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/644167/EU
oaire.citation.issue23pt_PT
oaire.citation.titleHeliyonpt_PT
oaire.citation.volume10pt_PT
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2020
oaire.fundingStream3599-PPCDT
oaire.fundingStreamH2020
person.familyNameOliveira
person.familyNameCavaco
person.familyNameFigueira
person.familyNameNapoleão
person.familyNameNeves
person.familyNameCastanho
person.givenNameFilipa
person.givenNameMarco
person.givenNameTiago
person.givenNamePatrícia
person.givenNameVera
person.givenNameMiguel
person.identifier1069324
person.identifier953259
person.identifier.ciencia-id1412-63B8-7494
person.identifier.ciencia-idC11E-8BE9-A97F
person.identifier.ciencia-id671C-1860-A160
person.identifier.orcid0000-0001-8816-9932
person.identifier.orcid0000-0002-0938-9038
person.identifier.orcid0000-0002-0813-0745
person.identifier.orcid0000-0002-5896-1440
person.identifier.orcid0000-0002-2989-7208
person.identifier.orcid0000-0001-7891-7562
person.identifier.ridO-2176-2018
person.identifier.scopus-author-id8872775500
person.identifier.scopus-author-id26537945300
person.identifier.scopus-author-id56605575600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3888b418-93b3-4355-85e5-16c4042e3b48
relation.isAuthorOfPublicationce7bbed1-4210-464f-93ac-f270ca7d2140
relation.isAuthorOfPublicationc65d67d4-bb09-45c4-be75-376e1f048665
relation.isAuthorOfPublicationc74643c7-434c-4310-9b98-2fcaa9fdd4b3
relation.isAuthorOfPublicationf62bb731-3f4d-4eaf-bc6b-dd379f6c2327
relation.isAuthorOfPublicationf5e46f85-fabf-450a-9ee2-1c7b59410892
relation.isAuthorOfPublication.latestForDiscoveryce7bbed1-4210-464f-93ac-f270ca7d2140
relation.isProjectOfPublicationf5fbfaa0-4654-4e9e-8d71-7bf0ed5539d5
relation.isProjectOfPublication4a2cb564-fe3e-4dae-9672-aef852dc23c3
relation.isProjectOfPublication10852667-c3b9-4751-b4ad-819c88b31a70
relation.isProjectOfPublication53a00574-2785-453c-bd31-f54e9661b033
relation.isProjectOfPublicationde0ab284-92e7-489b-9316-562e1995a110
relation.isProjectOfPublication.latestForDiscovery4a2cb564-fe3e-4dae-9672-aef852dc23c3

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
vCPP2319.pdf
Tamanho:
5.77 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.2 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

FM - Artigos em Revistas Internacionais