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Leaf morphoanatomy of portuguese autoctones white grapevine cultivars of different geographical origin

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The knowledge of grapevine varieties leaf morphoanatomy is an important tool to understand the taxa ability to adapt and produce under biotic and abiotic stresses. Aiming to characterize and discriminate between four white Portuguese grapevine (Vitis vinifera subsp. vinifera) varieties – ‘Alvarinho’ (Al), ‘Arinto’ (Ar), ‘Encruzado’ (En) and ‘Viosinho’ (Vi) - from different Winegrowing Regions (Vinhos Verdes, Lisboa, Dão and Douro, respectively), grown side by side in field conditions -, leaf morphoanatomic characteristics were studied under light (LM) and scanning electron microscopy (SEM). The individual primary leaf area revealed significant differences between all cultivars, with the highest value presented by ‘Arinto’ and the lowest by ‘Viosinho’, while ‘Encruzado’ and “Alvarinho” gave intermediate values. Nevertheless, no significant differences were detected on leaf specific dry weight which can be explained by the quite different mesophyll structure. ‘Arinto’ presented the lowest values for total thickness of the lamina, thickness of palisade and spongy parenchyma. The length and thickness of upper and lower epidermal cells of the four cultivars were similar. Under SEM magnification three types of stomata were identified in all the studied genotypes: sunken, at the same level, and raised above the other epidermal cells. No significant differences were registered between cultivars for same-level and sunken stomata values and for stomatal density. ‘Alvarinho’ showed the highest percentage of total for raised-above stomata and ‘Viosinho’ the lowest values. In conclusion, the data indicate some differences in leaf morphoanatomy between grapevine cultivars. Are these grapevine leaf traits differences – e.g. stomata type and mesophyll structure – involved in the differential behavior observed under field conditions? Further studies are needed

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Xth International Terroir Congress, Tokaj, Hungary, 2014

Keywords

grapevine leaf epidermis stomata mesophyll

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