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Advisor(s)
Abstract(s)
In order to investigate the effect of a dietary amino acid mixture supplementation in
lipopolysaccharide (LPS)-challenged weaned piglets, twenty-seven 28-day-old (8.2 1.0 kg) newly
weaned piglets were randomly allocated to one of three experimental treatments for five weeks. Diet
1: a CTRL treatment. Diet 2: an LPS treatment, where piglets were intraperitoneally administered
LPS (25 g/kg) on day 7. Diet 3: an LPS+MIX treatment, where piglets were intraperitoneally
administered LPS on day 7 and fed a diet supplemented with a mixture of 0.3% of arginine, branchedchain
amino acids (leucine, valine, and isoleucine), and cystine (MIX). Blood samples were drawn
on day 10 and day 35, and serum was analysed for selected chemical parameters and proteomics.
The LPS and LPS+MIX groups exhibited an increase in haptoglobin concentrations on day 10. The LPS group showed an increased cortisol concentration, while this concentration was reduced in
the LPS+MIX group compared to the control group. Similarly, the LPS+MIX group showed a
decreased haptoglobin concentration on day 35 compared to the two other groups. Immunoglobulin
concentrations were affected by treatments. Indeed, on day 10, the concentrations of IgG and
IgM were decreased by the LPS challenge, as illustrated by the lower concentrations of these two
immunoglobulins in the LPS group compared to the control group. In addition, the supplementation
with the amino acid mixture in the LPS+MIX further decreased IgG and increased IgM concentrations
compared to the LPS group. Although a proteomics approach did not reveal important alterations in
the protein profile in response to treatments, LPS-challenged piglets had an increase in proteins linked
to the immune response, when compared to piglets supplemented with the amino acid mixture.
Overall, data indicate that LPS-challenged piglets supplemented with this amino acid mixture are
more protected against the detrimental effects of LPS
Description
Keywords
piglets amino acids immunity challenge
Pedagogical Context
Citation
Prates, J.A.M.; Freire, J.P.B.; de Almeida, A.M.; Martins, C.; Ribeiro, D.M.; Osório, H.; Pinho, M.A.S.; Lopes, P.A.; Correia, J.M.J.; Pinto, R.M.A.; et al. Influence of Dietary Supplementation with an Amino Acid Mixture on Inflammatory Markers, Immune Status and Serum Proteome in LPS-ChallengedWeaned Piglets. Animals 2021, 11, 1143
Publisher
MDPI