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Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins

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dc.contributor.authorBrito, Paula
dc.contributor.authorAntunes, Fernando
dc.date.accessioned2022-02-16T14:34:46Z
dc.date.available2022-02-16T14:34:46Z
dc.date.issued2014
dc.descriptionCopyright © 2014 Brito and Antunes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.pt_PT
dc.description.abstractThe lack of kinetic data concerning the biological effects of reactive oxygen species is slowing down the development of the field of redox signaling. Herein, we deduced and applied equations to estimate kinetic parameters from typical redox signaling experiments. H2O2-sensing mediated by the oxidation of a protein target and the switch-off of this sensor, by being converted back to its reduced form, are the two processes for which kinetic parameters are determined. The experimental data required to apply the equations deduced is the fraction of the H2O2 sensor protein in the reduced or in the oxidized state measured in intact cells or living tissues after exposure to either endogenous or added H2O2. Either non-linear fittings that do not need transformation of the experimental data or linearized plots in which deviations from the equations are easily observed can be used. The equations were shown to be valid by fitting to them virtual time courses simulated with a kinetic model. The good agreement between the kinetic parameters estimated in these fittings and those used to simulate the virtual time courses supported the accuracy of the kinetic equations deduced. Finally, equations were successfully tested with real data taken from published experiments that describe redox signaling mediated by the oxidation of two protein tyrosine phosphatases, PTP1B and SHP-2, which are two of the few H2O2-sensing proteins with known kinetic parameters. Whereas for PTP1B estimated kinetic parameters fitted in general the present knowledge, for SHP-2 results obtained suggest that reactivity toward H2O2 as well as the rate of SHP-2 regeneration back to its reduced form are higher than previously thought. In conclusion, valuable quantitative kinetic data can be estimated from typical redox signaling experiments, thus improving our understanding about the complex processes that underlie the interplay between oxidative stress and redox signaling responses.pt_PT
dc.description.sponsorshipSupported by Fundação para a Ciência e a Tecnologia (FCT), Portugal (PEst-OE/QUI/UI0612/2013 and VIH/SAU/0020/2011).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFront Chem. 2014 Oct 2;2:82pt_PT
dc.identifier.doi10.3389/fchem.2014.00082pt_PT
dc.identifier.eissn2296-2646
dc.identifier.urihttp://hdl.handle.net/10451/51349
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontierspt_PT
dc.relationArtificial Zinc-Finger transcription factors libraries: Identification of HIV-1 cellular restriction factors and Therapeutic application in HIV-1 infection.
dc.relation.publisherversionhttps://www.frontiersin.org/journals/chemistry#pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPTP1Bpt_PT
dc.subjectSHP-2pt_PT
dc.subjectKineticspt_PT
dc.subjectProtein tyrosine phosphatasespt_PT
dc.subjectRate constantpt_PT
dc.subjectRedox regulationpt_PT
dc.subjectRedox signalingpt_PT
dc.titleEstimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteinspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPEst-OE/QUI/UI0612/2013
oaire.awardNumberVIH/SAU/0020/2011
oaire.awardTitleArtificial Zinc-Finger transcription factors libraries: Identification of HIV-1 cellular restriction factors and Therapeutic application in HIV-1 infection.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FQUI%2FUI0612%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/VIH%2FSAU%2F0020%2F2011/PT
oaire.citation.titleFrontiers in Chemistrypt_PT
oaire.citation.volume2pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameMatos de Brito
person.familyNameNunes Antunes
person.givenNamePaula
person.givenNameFernando José
person.identifier.ciencia-id7F16-5FB3-2395
person.identifier.ciencia-id711F-5E50-5F05
person.identifier.orcid0000-0002-0105-6968
person.identifier.orcid0000-0002-4192-2840
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf6975fda-1821-4c5f-affa-350b25c73325
relation.isAuthorOfPublication07693697-d50e-472d-9ac3-a737037c074b
relation.isAuthorOfPublication.latestForDiscoveryf6975fda-1821-4c5f-affa-350b25c73325
relation.isProjectOfPublicationcd502189-9837-408c-b975-66fdff720ac9
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