Logo do repositório
 
Publicação

Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs

2019-12-18Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorMartinez, CA
dc.contributor.authorCambra, JM
dc.contributor.authorParrilla, I
dc.contributor.authorRoca, J
dc.contributor.authorFerreira-Dias, Graça
dc.contributor.authorPallares, FJ
dc.contributor.authorLucas, X
dc.contributor.authorVazquez, JM
dc.contributor.authorMartinez, EA
dc.contributor.authorGil, MA
dc.date.accessioned2021-10-06T15:49:54Z
dc.date.available2021-10-06T15:49:54Z
dc.date.issued2019-12-18
dc.descriptionResearch Areas: Veterinary Sciencespt_PT
dc.description.abstractABSTRACT - Background: Seminal plasma (SP) promotes sperm survival and fertilizing capacity, and potentially affects embryo development, presumably via specific signaling pathways to the internal female genital tract. Objectives: This study evaluated how heterologous SP, infused immediately before postcervical artificial insemination (AI) affected embryo development and the transcriptional pattern of the pig endometria containing embryos. Materials and Methods: Postweaning estrus sows (n = 34) received 40-mL intrauterine infusions of either heterologous pooled SP or Beltsville Thawing Solution (BTS; control) 30 min before AI of semen extended to 10% of homologous SR. Embryos (all sows) and endometrium samples (3 sows/group) were removed during laparotomy 6 days after the infusion of SP or BTS to morphologically evaluate the embryos to determine their developmental stage and to analyze the endometrial transcriptome using microarrays (PORGENE 1.0 ST GeneChip array, Affymetrix) followed by qPCR for further validation. Results: Embryo viability was equal between the groups (similar to 93%), but embryo development was significantly (P < 0.05) more advanced in the SP-treated group compared to control. A total of 1,604 endometrium transcripts were differentially expressed in the SP group compared to the control group. An enrichment analysis showed an overrepresentation of genes and pathways associated with the immune response, cytokine signaling, cell cycle, cell adhesion, and hormone response, among others. Conclusions: SP infusions prior to AI positively impacted the preimplantation embryo development and altered the expression of the endometrial genes and pathways potentially involved in embryo development.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMartinez CA, Cambra JM, Parrilla I, Roca J, Ferreira-Dias G, Pallares FJ, Lucas X, Vazquez JM, Martinez EA, Gil MA. 2019. Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs. Frontiers in Veterinary Science. 6:465.pt_PT
dc.identifier.doi10.3389/fvets.2019.00465pt_PT
dc.identifier.eissn2297-1769
dc.identifier.urihttp://hdl.handle.net/10400.5/22150
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherArumugam Kumaresan, National Dairy Research Institute (ICAR), Indiapt_PT
dc.relationAGL2015-69735-R (Ministry of Economy and Competitiveness (Madrid, Spain)/the European Regional Development Fund)pt_PT
dc.relation19892/GERM/15pt_PT
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fvets.2019.00465/fullpt_PT
dc.subjectSeminal plasmapt_PT
dc.subjectendometriumpt_PT
dc.subjecttranscriptomept_PT
dc.subjectembryopt_PT
dc.subjectpreimplantationpt_PT
dc.subjectpigpt_PT
dc.titleSeminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue465pt_PT
oaire.citation.titleFrontiers in Veterinary Sciencept_PT
oaire.citation.volumev. 6pt_PT
person.familyNameLeitão Ferreira-Dias
person.givenNameGraça Maria
person.identifier.ciencia-idF51C-7F45-C720
person.identifier.orcid0000-0003-0622-6513
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0fb8ec33-7ee1-482b-bdcb-eff773226f90
relation.isAuthorOfPublication.latestForDiscovery0fb8ec33-7ee1-482b-bdcb-eff773226f90

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Seminal plasma modifies the transcriptional pattern of the endometrium and advances embryo development in pigs.pdf
Tamanho:
3.12 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CIISA - Artigos em revistas internacionais