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An effective culturomics approach to study the gut microbiota of mammals

2020-12Journal article Open access
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dc.contributor.authorPereira, André C.
dc.contributor.authorCunha, Mónica V.
dc.date.accessioned2021-01-29T19:58:53Z
dc.date.available2021-12-01T01:30:35Z
dc.date.issued2020-12
dc.description.abstractThe microbial characterization of the mammal's gut is an emerging research area, wherein culturomics methodologies applied to human samples are transposed to the animal context without improvement. In this work, using Egyptian mongoose as a model, we explore wet bench conditions to define an effective experimental design based on culturomics and DNA barcoding with potential application to different mammal species. After testing a battery of solid media and enrichments, we show that YCFA-based media, in aerobic and anaerobic conditions, together with PDA supplemented with chloramphenicol, are sufficient to maximize bacterial and fungal microbiota diversity. The pasteurization of the sample enrichment before cultivation is central to gain insight into sporogenic communities. We suggest the application of this optimized culturomics strategy to accurately expand knowledge on the microbial richness of mammals' gut, maximizing the application of common laboratory resources, without dramatic time and consumables expenditure but with high resolution of microbial landscapes. The analysis of ten fecal samples proved adequate to assess the core gastrointestinal microbiota of the mesocarnivore under analysis. This approach may empower most microbiology laboratories, particularly the veterinary, to perform studies on mammal's microbiota, and, in contrast with metagenomics, enabling the recovery of live bacteria for further studies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAndré C. Pereira, Mónica V. Cunha, An effective culturomics approach to study the gut microbiota of mammals, Research in Microbiology, Volume 171, Issue 8, 2020, Pages 17 290-300, ISSN 0923-2508, https://doi.org/10.1016/j.resmic.2020.09.001.pt_PT
dc.identifier.doi10.1016/j.resmic.2020.09.001pt_PT
dc.identifier.urihttp://hdl.handle.net/10451/46040
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationUID/BIA/00329/2020pt_PT
dc.relationUID/Multi/04046/2020pt_PT
dc.relationIncorporating the genomic signature of environmental Mycobacterium bovis into transmission models of animal TB under a phylodynamics conceptual framework
dc.titleAn effective culturomics approach to study the gut microbiota of mammalspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleIncorporating the genomic signature of environmental Mycobacterium bovis into transmission models of animal TB under a phylodynamics conceptual framework
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F136557%2F2018/PT
oaire.citation.endPage300pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage290pt_PT
oaire.citation.titleResearch in Microbiologypt_PT
oaire.citation.volume171pt_PT
person.familyNameCunha
person.givenNameMonica V.
person.identifier348477
person.identifier.ciencia-id5616-E715-2F31
person.identifier.orcid0000-0003-0401-0276
person.identifier.ridD-5544-2011
person.identifier.scopus-author-id7102711216
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPeríodo de embargo de 12 meses de acordo com a editora Elsevierpt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa1b365bb-7421-4bf5-9cde-62b74c170e69
relation.isAuthorOfPublication.latestForDiscoverya1b365bb-7421-4bf5-9cde-62b74c170e69
relation.isProjectOfPublication26807cc9-7169-4693-b529-692be4f7abe8
relation.isProjectOfPublication.latestForDiscovery26807cc9-7169-4693-b529-692be4f7abe8

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