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Contribution of haptoglobin phenotypic variation to the presence of hyperhomocysteinemia in type 2 diabetics with and without angiopathy

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dc.contributor.authorFerreira, Isabel
dc.contributor.authorBicho, Manuel
dc.contributor.authorValente, Ana
dc.date.accessioned2025-04-04T11:42:07Z
dc.date.available2025-04-04T11:42:07Z
dc.date.issued2024
dc.description© The Author(s), under exclusive licence to Springer Nature Limited 2024pt_PT
dc.description.abstractBackground/aim: The genetic polymorphism of haptoglobin (Hp) has been associated with several cardiovascular risk factors, but a possible relationship between Hp phenotypic variation and increased levels of homocysteine (Hcy) and cysteine (Cy) is still unknown. The objective of this study is to evaluate the relationship between the Hp polymorphism and hyperhomocysteinemia (HHcy) and hypercysteinemia (HCy) in type 2 diabetics (T2D) with and without angiopathy (AGP). Methods: A case-control study was carried out on 293 adults: Group I (GI) - 75 subjects with T2D and AGP; Group II (GII) - 75 subjects with T2D without AGP; Group III (GIII) - 143 controls. Plasma levels of Hcy, Cy and vitamin B6 were measured by high performance liquid chromatography (HPLC) and vitamins B9 and B12 determined by electrochemiluminescence (ECL). The Hp polymorphism was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and peroxidase staining. The results were analyzed in SPSS®, version 26.0 with a significance of 95%. Results: Mean Hcy concentrations were significantly lower in carriers of the Hp2-2 phenotype (6.14 µM; p = 0.046) compared to the other genotypes. The presence of Hp2-1 is associated with an approximately 3.3 times greater probability of occurrence of HHcy (p = 0.015) and 3.7 times greater probability occurrence of HCy (p = 0.021) in T2D with AGP. Conclusion: The presence of the Hp2-1 phenotype is associated with the predisposition of HHcy and HCy in individuals with T2D and AGP, possibly through a positive heterosis mechanism. Carriers of the Hp2-2 phenotype appear to have a greater activation of the transsulfuration pathway in the Hcy cycle and consequent protection for its accumulation.pt_PT
dc.description.sponsorshipThis study was finanially supported by the research project PIC/IC/82957/2007 from Foundation for Science and Technology (FCT) and by Instituto Nacional de Saúde Doutor Ricardo Jorge, I.P. (Lisboa, Portugal). This study had also the financial support of the FCT for Environmental Health Institute (references UIDB/04295/2020 and UIDP/04295/2020). Ana Valente acknowledges the financial support received from FCT, through the PhD grant (SFRH/BD/16166/2004/5E4M).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEur J Clin Nutr. 2025 Mar;79(3):266-272pt_PT
dc.identifier.doi10.1038/s41430-024-01524-7pt_PT
dc.identifier.eissn1476-5640
dc.identifier.issn1476-5640
dc.identifier.urihttp://hdl.handle.net/10400.5/100004
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationSFRH/BD/16166/2004/5E4Mpt_PT
dc.relationEnvironmental Health Institute
dc.relation.publisherversionhttps://www.nature.com/ejcn/pt_PT
dc.titleContribution of haptoglobin phenotypic variation to the presence of hyperhomocysteinemia in type 2 diabetics with and without angiopathypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEnvironmental Health Institute
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5646-ICCMS/PIC%2FIC%2F82957%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F04295%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04295%2F2020/PT
oaire.citation.endPage272pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage266pt_PT
oaire.citation.titleEuropean Journal of Clinical Nutritionpt_PT
oaire.citation.volume79pt_PT
oaire.fundingStream5646-ICCMS
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Base
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBicho
person.familyNameValente
person.givenNameManuel
person.givenNameAna
person.identifier.ciencia-id9512-8D22-1B28
person.identifier.ciencia-idFE1E-5A9B-EC3D
person.identifier.orcid0000-0002-5773-5687
person.identifier.orcid0000-0003-4039-4326
person.identifier.scopus-author-id7004153755
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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