Publicação
Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescence
| dc.contributor.author | Santos, Andrea Martins dos | |
| dc.date.accessioned | 2014-03-27T14:45:41Z | |
| dc.date.available | 2014-03-27T14:45:41Z | |
| dc.date.issued | 2012 | |
| dc.description | © Springer Science+Business Media, LLC 2011. | eng |
| dc.description.abstract | The emission behaviour of Aequorea green fluorescent protein (A-GFP) chromophore, in both neutral (N) and anionic (A) form, was studied in the temperature range from 20 °C to 75 °C and at pH=7. Excitation wavelengths of 399 nm and 476 nm were applied to probe the N and A forms environment, respectively. Both forms exhibit distinct fluorescence patterns at high temperature values. The emission quenching rate, following a temperature increase, is higher for the chromophore N form as a result of the hydrogen bond network weakening. The chromophore anionic form emission maximum is red shifted, upon temperature increase, due to a charge transfer process occurring after A form excitation. | eng |
| dc.description.sponsorship | The author acknowledges financial support from Fundação para a Ciência e a Tecnologia through grant SFRH/BPD/26821/2006. Dr. V. Subramanian (University of Twente,NL) for providing the A-GFP gene, and Dr. F. Mergulhão (University of Porto, PT) for the support given on A-GFP expression and purification. | eng |
| dc.identifier.citation | J Fluoresc (2012) 22:151–154 | por |
| dc.identifier.issn | 1053-0509 | |
| dc.identifier.uri | http://dx.doi.org/10.1007/s10895-011-0941-0 | |
| dc.identifier.uri | http://hdl.handle.net/10451/10789 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Springer | por |
| dc.relation.publisherversion | The definitive version is available at http://link.springer.com/ | eng |
| dc.subject | Green fluorescent protein | eng |
| dc.subject | Chromophore | eng |
| dc.subject | Fluorescence | eng |
| dc.subject | Temperature | eng |
| dc.title | Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescence | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 154 | por |
| oaire.citation.startPage | 151 | por |
| oaire.citation.title | Journal of Fluorescence | eng |
| oaire.citation.volume | 22 | por |
| rcaap.rights | closedAccess | por |
| rcaap.type | article | por |