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Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets

2024-02Journal article Open access
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dc.contributor.authorRibeiro, David Miguel
dc.contributor.authorCoelho, Diogo
dc.contributor.authorCosta, Mónica
dc.contributor.authorCarvalho, Daniela
dc.contributor.authorLeclercq, Céline C.
dc.contributor.authorRenaut, Jenny
dc.contributor.authorFreire, João
dc.contributor.authorAlmeida, André
dc.contributor.authorPrates, José António Mestre
dc.date.accessioned2025-07-30T11:18:42Z
dc.date.available2025-07-30T11:18:42Z
dc.date.issued2024-02
dc.description.abstractSeaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics (RNA-seq) and proteomics (LC–MS) approach. Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrient availability, leading to protein degradation through the proteasome (PSMD2), with resulting amino acids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulation may have contributed to increased oxidative stress, evident from elevated levels of antioxidant proteins like catalase, as a response to maintaining tissue homeostasis. The upregulation of the gene AQP7, associated with the osmotic stress response, further supports these findings. Consequently, an increase in chaperone activity, including HSP90, was required to repair damaged proteins. Our results suggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactuca alone, potentially due to side effects of cell wall degradation during digestion.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRibeiro, David Miguel, et al. «Integrated Transcriptomics and Proteomics Analysis Reveals Muscle Metabolism Effects of Dietary Ulva Lactuca and Ulvan Lyase Supplementation in Weaned Piglets». Scientific Reports, vol. 14, n.o 1, fevereiro de 2024, p. 4589. https://doi.org/10.1038/s41598-024-55462-2.pt_PT
dc.identifier.doi10.1038/s41598-024-55462-2pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/102540
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNaturept_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationLaboratory for Sustainable Land Use and Ecosystem Services
dc.relation.publisherversionwww.nature.com/scientificreportspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectWeaned pigletpt_PT
dc.subjectUlva lactucapt_PT
dc.subjectMusclept_PT
dc.subjectTranscriptomicspt_PT
dc.subjectProteomicspt_PT
dc.titleIntegrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned pigletspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleLaboratory for Sustainable Land Use and Ecosystem Services
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017/PTDC%2FCAL-ZOO%2F30238%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F143992%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0059%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F04129%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0092%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage4589pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume14pt_PT
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Base
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRibeiro Viera Santos
person.familyNamePires Carvalho
person.familyNameBengala Freire
person.familyNameMartinho de Almeida
person.givenNameDavid Miguel
person.givenNameDaniela Filipa
person.givenNameJoão Pedro
person.givenNameAndré
person.identifier.ciencia-id2714-8393-F2A3
person.identifier.ciencia-id7915-4FF9-FEC8
person.identifier.ciencia-id871A-F6C8-60FE
person.identifier.ciencia-id1E14-92CC-B163
person.identifier.orcid0000-0002-0990-7739
person.identifier.orcid0000-0001-8419-8701
person.identifier.orcid0000-0002-1811-4378
person.identifier.orcid0000-0002-7810-3988
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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