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Identification and determination of the viability of Giardia lamblia cysts and Cryptosporidium parvum and Cryptosporidium hominis oocysts in human fecal and water supply samples by fluorescent in situ hybridization (FISH) and monoclonal antibodies

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dc.contributor.authorLemos, Vanessa
dc.contributor.authorGraczyk, Thaddeus K
dc.contributor.authorAlves, Margarida
dc.contributor.authorLobo, Maria Luísa
dc.contributor.authorSousa, Maria C
dc.contributor.authorAntunes, Francisco
dc.contributor.authorMatos, Olga
dc.date.accessioned2025-07-31T10:37:48Z
dc.date.available2025-07-31T10:37:48Z
dc.date.issued2005
dc.description© Springer-Verlag 2005pt_PT
dc.description.abstractIn the present study, fluorescent in situ hybridization (FISH) and monoclonal antibodies (MAbs) were evaluated for species-specific detection and viability determination of Giardia lamblia, Cryptosporidium parvum, and Cryptosporidium hominis in human fecal and water supply samples. A total of 50 fecal human samples positive for G. lamblia cysts, 38 positive for C. parvum, and 23 positive for C. hominis were studied. Also, 18 water supply samples positive for Giardia spp. and Cryptosporidium spp. by the United States Environmental Protection Agency (USEPA) Method 1623 were studied by FISH and fluorescein isothiocyanate (FITC)-conjugated MAbs. Eighteen percent of the fecal samples parasitologically positive for G. lamblia presented viable and nonviable cysts, and 5% of those positive for Cryptosporidium spp. presented viable and nonviable oocysts. Of the 18 water supply samples analyzed, 6 (33%) presented Giardia spp. viable and nonviable cysts and 2 (11%) presented viable and nonviable Cryptosporidium spp. oocysts. G. lamblia identification was confirmed by polymerase chain reaction (PCR) and sequencing of the beta-giardin gene in the fecal and water samples found positive by FISH and FITC-conjugated MAbs. C. parvum and Cryptosporidium muris were identified, by PCR and sequencing of the small subunit of ribosomal RNA gene, in seven and one water samples, respectively. Our results confirm that this technique enables simultaneous visualization, species-specific identification, and viability determination of the organisms present in human fecal and water supply samples.pt_PT
dc.description.sponsorshipThis work was supported by the Project POCTI/ESP/46369/2002 from Fundação para a Ciência e Tecnologia (FCT)/EU/FSE/FEDER and by a NATO Collaborative Linkage Grant, CLG 979765pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationParasitol Res. 2005 Dec;98(1):48-53pt_PT
dc.identifier.doi10.1007/s00436-005-0018-6pt_PT
dc.identifier.eissn1432-1955
dc.identifier.issn0932-0113
dc.identifier.urihttp://hdl.handle.net/10400.5/102574
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationStudy of the Transmission Dynamics of Cryptosporidium Using Molecular Analysis of isolates from Humans, Animals and Water
dc.relation.publisherversionhttps://link.springer.com/journal/436pt_PT
dc.subjectGiardia lambliapt_PT
dc.subjectCryptosporidium parvumpt_PT
dc.subjectCryptosporidium hominispt_PT
dc.subjectDetectionpt_PT
dc.subjectViabilitypt_PT
dc.subjectWater supply samplespt_PT
dc.subjectFISHpt_PT
dc.subjectMAbspt_PT
dc.titleIdentification and determination of the viability of Giardia lamblia cysts and Cryptosporidium parvum and Cryptosporidium hominis oocysts in human fecal and water supply samples by fluorescent in situ hybridization (FISH) and monoclonal antibodiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStudy of the Transmission Dynamics of Cryptosporidium Using Molecular Analysis of isolates from Humans, Animals and Water
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FESP%2F46369%2F2002/PT
oaire.citation.endPage53pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage48pt_PT
oaire.citation.titleParasitology Researchpt_PT
oaire.citation.volume98pt_PT
oaire.fundingStreamPOCI
person.familyNameAntunes
person.familyNameGuerreiro de Matos
person.givenNameFrancisco
person.givenNameOlga Maria
person.identifier.ciencia-id751F-FE84-CCF2
person.identifier.orcid0000-0001-7932-1154
person.identifier.orcid0000-0001-5793-7716
person.identifier.scopus-author-id7006648941
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa28493a5-9101-4173-8863-74ee946d6ced
relation.isAuthorOfPublicationbe64fd9b-8fc7-450b-b84d-c667e7d78760
relation.isAuthorOfPublication.latestForDiscoverybe64fd9b-8fc7-450b-b84d-c667e7d78760
relation.isProjectOfPublication3b0d9986-644c-4bbc-90f1-bfd30cdf050d
relation.isProjectOfPublication.latestForDiscovery3b0d9986-644c-4bbc-90f1-bfd30cdf050d

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