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Publicação
Identificação de genes expressos durante a interacção cv. florina - v. inaequalis por differential display-rt-pcr
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Orientador(es)
Resumo(s)
In order to identify genes that are specifically
regulated during the expression of the
resistance mechanism of the apple cv. Florina
towards the apple scab fungus, the genetic
expression pattern in plants inoculated with
a conidial suspension of Venturia inaequalis
was compared with the expression pattern in
plants inoculated with water, by differential
display RT-PCR. Amplified products derived
from the combination of one oligodT (dTVG)
with 20 10-mer primers were separated in
polyacrylamide denaturing gel and detected
with silver nitrate. 14 cDNA fragments that
were only visible in the sample inoculated
with fungal suspension, or more abundant
in this one, were purified from the gel for
reamplification and cloning. Three fragments
were already sequenced and one sequence
showed very strong homology with a plant
phytochelatin synthetase, that might be involved
in stress responses. Differential accumulation
of this cDNA upon fungal inoculation
shall be verified by reverse-transcription
PCR with specific primers.
Descrição
Palavras-chave
apple scab differential display-rt-pcr Florina phytochelatin synthetase expressão diferencial pedrado macieira fitoquelatina sintetase
Contexto Educativo
Citação
"Revista de Ciências Agrárias". ISSN 0871-018X. 31:2 (2008) 139-147
Editora
Sociedade de Ciências Agrárias de Portugal