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RNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicing

2018Journal article Open access
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dc.contributor.authorNojima, Takayuki
dc.contributor.authorRebelo, Kenny
dc.contributor.authorGomes, Tomás
dc.contributor.authorGrosso, Ana Rita
dc.contributor.authorProudfoot, Nicholas J.
dc.contributor.authorCarmo-Fonseca, Maria
dc.date.accessioned2021-07-28T14:20:13Z
dc.date.available2021-07-28T14:20:13Z
dc.date.issued2018
dc.description© 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractThe highly intronic nature of protein coding genes in mammals necessitates a co-transcriptional splicing mechanism as revealed by mNET-seq analysis. Immunoprecipitation of MNase-digested chromatin with antibodies against RNA polymerase II (Pol II) shows that active spliceosomes (both snRNA and proteins) are complexed to Pol II S5P CTD during elongation and co-transcriptional splicing. Notably, elongating Pol II-spliceosome complexes form strong interactions with nascent transcripts, resulting in footprints of approximately 60 nucleotides. Also, splicing intermediates formed by cleavage at the 5' splice site are associated with nearly all spliced exons. These spliceosome-bound intermediates are frequently ligated to upstream exons, implying a sequential, constitutive, and U12-dependent splicing process. Finally, lack of detectable spliced products connected to the Pol II active site in human HeLa or murine lymphoid cells suggests that splicing does not occur immediately following 3' splice site synthesis. Our results imply that most mammalian splicing requires exon definition for completion.pt_PT
dc.description.sponsorshipThis work was supported by funding to N.J.P. (Wellcome Trust Investigator Award [107928/Z/15/Z] and ERC Advanced [339270] grants) and to M.C.-F. (Fundação Ciência e Tecnologia, Portugal; grant PTDC/BEX-BCM/5899/2014).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMol Cell. 2018 Oct 18;72(2):369-379.e4pt_PT
dc.identifier.doi10.1016/j.molcel.2018.09.004pt_PT
dc.identifier.eissn1097-4164
dc.identifier.issn1097-2765
dc.identifier.urihttp://hdl.handle.net/10451/49190
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation107928/Z/15/Zpt_PT
dc.relationPolicing mammalian transcriptomes: regulation of long ncRNA synthesis by transcriptional termination, gene loops and R-loops.
dc.relationMaking sense of antisense transcription
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/molecular-cellpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectRNA polymerase IIpt_PT
dc.subjectSpliceosomept_PT
dc.subjectCo-transcriptional splicingpt_PT
dc.subjectExon definitionpt_PT
dc.subjectmNET-MSpt_PT
dc.subjectmNET-seqpt_PT
dc.subjectPhospho-serine 5 CTDpt_PT
dc.subjectSplicing intermediatespt_PT
dc.titleRNA polymerase II phosphorylated on CTD serine 5 interacts with the spliceosome during co-transcriptional splicingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePolicing mammalian transcriptomes: regulation of long ncRNA synthesis by transcriptional termination, gene loops and R-loops.
oaire.awardTitleMaking sense of antisense transcription
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/339270/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBEX-BCM%2F5899%2F2014/PT
oaire.citation.endPage379pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage369pt_PT
oaire.citation.titleMolecular Cellpt_PT
oaire.citation.volume72pt_PT
oaire.fundingStreamFP7
oaire.fundingStream3599-PPCDT
person.familyNameGomes
person.familyNameGrosso
person.familyNameCarmo-Fonseca
person.givenNameTomás
person.givenNameAna Rita
person.givenNameMaria
person.identifierI-6656-2013
person.identifier.ciencia-id3E11-841C-AE1B
person.identifier.ciencia-id3D18-571E-133A
person.identifier.ciencia-idB31F-0435-0753
person.identifier.orcid0000-0003-1333-0191
person.identifier.orcid0000-0001-6974-4209
person.identifier.orcid0000-0002-3402-7143
person.identifier.scopus-author-id26639262500
person.identifier.scopus-author-id7007128195
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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