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Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans

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dc.contributor.authorMarques, Cátia Filipa Saraiva
dc.contributor.authorBelas, Adriana
dc.contributor.authorAboim, Catarina
dc.contributor.authorTrigueiro, Graça
dc.contributor.authorCavaco-Silva, Patrícia
dc.contributor.authorGama, Luis
dc.contributor.authorPomba, C.
dc.date.accessioned2021-10-06T15:08:42Z
dc.date.available2021-10-06T15:08:42Z
dc.date.issued2019-01
dc.descriptionResearch Areas: Microbiology. Veterinary Sciencespt_PT
dc.description.abstractABSTRACT - Proteus mirabilis is a major cause of urinary tract infection (UTI) in humans and companion animals. This study aimed to evaluate the antimicrobial resistance, virulence and clonal relatedness of P. mirabilis isolated from dogs, cats and humans with UTI. P. mirabilis isolated from companion animals (N = 107) and humans (N = 76) with UTI were compared by PFGE analysis after overnight Nod macro-restriction using Dice/UPGMA with a 1.5% tolerance. Strains were characterized for antimicrobial resistance by disk diffusion. Twenty-four resistance genes and four virulence genes were screened by PCR. Thirty-nine clusters (similarity > 80%) and 73 single pulse-types were detected. Nine clusters included P. mirabilis isolated from community and hospital patients, including strains with 100% similarity. A high number of clusters (43.6%, n = 17/39) included strains from companion animals and humans. Similarity between some companion animal and human strains varied between 80-100%. One strain from a dog was 100% similar to one human community-acquired P. mirabilis. One P. mirabilis from a cat was found to be 94.7% and 92.4% similar to community and hospital patient strains, respectively. P. mirabilis CMY-2-producers did not cluster all together. Nevertheless, cluster C36 included five P. mirabilis from companion animals (similarity 85.8%-95.7%), of which, four (80%) were multidrug-resistant CMY-2-producers. This study shows that companion animals and humans become infected with closely related P. mirabilis strains. The high number of clusters containing companion animals and human strains points to the zoonotic nature of P. mirabilis. These results underline the potential role of companion animals as reservoirs and in the dissemination of uropathogenic P. mirabilis to humans and vice versa.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMarques C, Belas A, Aboim C, Trigueiro G, Cavaco-Silva P, Gama LT, Pomba C. 2019. Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans. Veterinary Microbiology. 228(1):77-82pt_PT
dc.identifier.doi10.1016/j.vetmic.2018.10.015pt_PT
dc.identifier.issn0378-1135
dc.identifier.urihttp://hdl.handle.net/10400.5/22146
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherELSEVIERpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0378113518308733?via%3Dihubpt_PT
dc.subjectProteus mirabilispt_PT
dc.subjectUrinary tract infectionpt_PT
dc.subjectCompanion animalpt_PT
dc.subjectHumanpt_PT
dc.subjectClonal relatednesspt_PT
dc.titleClonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humanspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FCVT%2F00276%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/148846/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/48521/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F77886%2F2011/PT
oaire.citation.endPage82pt_PT
oaire.citation.startPage77pt_PT
oaire.citation.titleVeterinary microbiologypt_PT
oaire.citation.volumev. 228pt_PT
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
oaire.fundingStreamSFRH
person.familyNameMarques
person.familyNameInácio Belas
person.familyNameGama
person.familyNameMatias Ferreira Pomba
person.givenNameCátia
person.givenNameAdriana de Jesus
person.givenNameLuis
person.givenNameMaria Constança
person.identifier.ciencia-id8015-B7EE-5FFC
person.identifier.ciencia-id4D1D-1718-6FFF
person.identifier.ciencia-idC71D-140F-E876
person.identifier.ciencia-idDA1D-20A8-7F3C
person.identifier.orcid0000-0001-9648-6380
person.identifier.orcid0000-0001-6564-6839
person.identifier.orcid0000-0003-3894-3488
person.identifier.orcid0000-0002-0504-6820
person.identifier.ridN-4172-2018
person.identifier.scopus-author-id55433369800
person.identifier.scopus-author-id36027387300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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